公开(公告)号：US11280781B2

公开(公告)日：2022-03-22

申请号：US16587062

申请日：2019-09-30

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo Hyun Lee ,  Nakwon Choi ,  Byung Chul Lee ,  Hyungbeen Lee ,  Mintack Oh

IPC: G01N33/543 ,  C12M1/00 ,  G01N29/02 ,  C12N15/10

Abstract: The present disclosure relates to an apparatus and a method for extracting genome, capable of acquiring a sufficient amount of genome for genetic analysis with high extraction efficiency, even with a small amount of target sample.

公开(公告)号：US12247910B2

公开(公告)日：2025-03-11

申请号：US17962066

申请日：2022-10-07

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Il-Joo Cho ,  Nakwon Choi ,  Hyogeun Shin

IPC: G01N15/1434 ,  F21V8/00 ,  G01N15/14 ,  G02B1/04

Abstract: A high resolution fluorescence imaging device includes a light guide plate having a fluid channel which allows a solution containing a fluorescent sample to pass; a light source disposed on a side of the light guide plate to irradiate light inward the light guide plate; an image sensor disposed under the light guide plate; and a thin film interposed between the light guide plate and the image sensor, wherein the thin film has a first surface having a plurality of pillars and a second surface, the pillars separate the light guide plate and the image sensor apart from each other, and the second surface is bonded to the light guide plate. According to an embodiment, since the light guide plate acts in place of a filter element, it is possible to manufacture in ultrasmall size and achieve multiple fluorescence imaging without replacing a filter.

公开(公告)号：US10030219B2

公开(公告)日：2018-07-24

申请号：US15011385

申请日：2016-01-29

Applicant: CHUNGBUK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION ,  KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  Hong-Jun Lee

Inventor： Sang-Hoon Cha ,  Hong-Jun Lee ,  Nakwon Choi ,  Hong-Nam Kim

IPC: A61F2/82 ,  C12M3/06 ,  C12N5/079 ,  C12M1/12 ,  C12M1/00 ,  C12N5/0793 ,  C12N5/0797

Abstract: Provided are a neurovascular unit (NVU)-on-a-chip and a method of fabricating the same, which 3-dimensionally integrates various human brain cells in a microfluidic platform by using a brain cell co-culture technique so as to simulate a similar environment to the human brain in vitro. The NVU-on-a-chip includes an extracellular matrix (ECM) simulation material (70) in a gel state; and at least one channel (75) which passes through the ECM simulation material (70) and perfuses a culture medium, in which the ECM simulation material (70) contains a plurality of types of human brain cells on an outer side of the channel (75), a brain microvessel endothelial cell lining (91) is formed on an inner side of the channel (75), and the plurality of types of human brain cells and the brain microvessel endothelial cell lining (91) contact each other through the channel (75) to simulate a blood brain barrier (BBB) of a human brain and a neurovascular unit (NVU) of the human brain including the BBB.

公开(公告)号：US11638575B2

公开(公告)日：2023-05-02

申请号：US16789881

申请日：2020-02-13

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Byung Chui Lee ,  Hyung Min Kim ,  Ki Joo Pahk ,  Hyunsu Lee ,  Nakwon Choi ,  Hong Nam Kim

IPC: A61B8/00 ,  A61B8/08 ,  B06B1/02 ,  A61N7/02 ,  A61N7/00

Abstract: An ultrasonic diagnosis and therapy apparatus according to an embodiment may include an ultrasound output unit including a plurality of ultrasound output elements, a circuit board that can be attached and detached through a connecting board connected to the ultrasound output unit to determine a function of the ultrasound output unit, and a control unit configured to control a setting value of each of the plurality of ultrasound output elements, wherein therapy and diagnosis functions are selectively or simultaneously implemented by changing the circuit board. With the ultrasonic diagnosis and therapy apparatus, it is possible to selectively or simultaneously implement the therapy and diagnosis functions by selectively mounting different types of circuit boards that determine the type and function of ultrasound outputted from the ultrasonic transducers.

公开(公告)号：US10281419B2

公开(公告)日：2019-05-07

申请号：US15105740

申请日：2016-03-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kyoseon Hwang ,  Nakwon Choi ,  Jinsik Kim ,  Hye Jin Kim ,  Yong Kyoung Yoo ,  Sang Yun Yeom ,  Woongsun Choi

IPC: G01N27/12 ,  G01N33/53 ,  G01N33/483 ,  G01N33/487 ,  G01N33/543

Abstract: A hydrogel-based interdigitated microelectrode biosensor is disclosed. The hydrogel-based interdigitated microelectrode biosensor includes: a first interdigitated microelectrode having a plurality of first protrusion electrodes arranged in a comb-like shape on a substrate; a second interdigitated microelectrode facing the first interdigitated microelectrode and having a plurality of second protrusion electrodes arranged in a comb-like shape on the substrate, the second protrusion electrodes being arranged alternately with the first protrusion electrodes of the first interdigitated microelectrode; and a hydrogel filled in a space between the first and second interdigitated microelectrodes arranged alternately with each other. The hydrogel is provided between the interdigitated microelectrodes such that the presence and concentration of a biological substance, such as a protein, are detected by measuring the impedance between the interdigitated microelectrodes.

公开(公告)号：US09061137B2

公开(公告)日：2015-06-23

申请号：US13688729

申请日：2012-11-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo Hyun Lee ,  Ji Yoon Kang ,  Nakwon Choi

IPC: A61N1/05 ,  A61L31/16 ,  A61L31/10 ,  A61B5/04

CPC classification number: A61N1/0556 ,  A61B5/04001 ,  A61B2562/0209 ,  A61B2562/125 ,  A61L31/10 ,  A61L31/16 ,  A61L2300/41 ,  A61L2300/43 ,  A61L2400/12 ,  A61L2420/08 ,  A61N1/0553

Abstract: A nerve electrode that is inserted into a living body and that is configured to attach to nerves is provided. The nerve electrode that is inserted into a living body and that is configured to attach to nerves includes: i) a flexible substrate; ii) a plurality of electrodes that are separately positioned on the flexible substrate; and iii) an insulating layer that is positioned at a separation space of the plurality of electrodes and that insulates the plurality of electrodes. The plurality of electrodes include i) at least one linear electrode, and ii) a planar electrode that is separated from the linear electrode. An anti-inflammatory drug transfer layer is positioned on the planar electrode.

Abstract translation: 提供了插入生物体并被配置为附着于神经的神经电极。 插入到生物体中并被配置为附着到神经的神经电极包括：i）柔性基底; ii）分别定位在柔性基板上的多个电极; 以及iii）绝缘层，其位于所述多个电极的分离空间处并使所述多个电极绝缘。 多个电极包括i）至少一个线状电极，以及ii）与线状电极分离的平面电极。 将抗炎药物转移层定位在平面电极上。

公开(公告)号：US09803167B2

公开(公告)日：2017-10-31

申请号：US14950710

申请日：2015-11-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Nakwon Choi ,  Eun-Mi Hur ,  Sohyun Kim ,  Sun-Kyoung Im ,  Eui Sung Yoon ,  Changjoon Justin Lee

IPC: B29C55/00 ,  C12M1/42 ,  B29C55/02 ,  B29C55/20 ,  B29C55/12 ,  C08J5/00 ,  A61L27/16 ,  A61L27/38 ,  A61L27/50

CPC classification number: C12M35/04 ,  A61L27/165 ,  A61L27/3804 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3834 ,  A61L27/50 ,  A61L2430/32 ,  B29C55/02 ,  B29C55/023 ,  B29C55/12 ,  B29C55/20 ,  C08J5/00

Abstract: The present disclosure relates to stretching apparatus and method for aligning microfibrils. Specifically, the present disclosure provides an apparatus for aligning microfibrils along a single direction, which includes: a first elastic substrate onto which a composition containing microfibrils is loaded; and a stretching module which stretches the width of the elastic substrate. In accordance with the apparatus the present disclosure, microfibrils or cells may be aligned along a particular direction simply by pulling and then releasing the elastic substrate. The present disclosure is also useful for culturing of the aligned cells because the physiological activity of the cells can be maintained and cytotoxicity can be prevented.

公开(公告)号：US20170211029A1

公开(公告)日：2017-07-27

申请号：US15011385

申请日：2016-01-29

Applicant: Hong-Jun Lee ,  CHUNGBUK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION ,  KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang-Hoon CHA ,  Hong-Jun Lee ,  Nakwon Choi ,  Hong-Nam Kim

IPC: C12M3/06 ,  C12N5/0797 ,  C12M1/00 ,  C12N5/0793 ,  C12N5/079 ,  C12M1/12

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M25/14 ,  C12M29/10 ,  C12M35/08 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0623 ,  C12N2502/08 ,  C12N2513/00 ,  C12N2533/90

Abstract: Provided are a neurovascular unit (NVU)-on-a-chip and a method of fabricating the same, which 3-dimensionally integrates various human brain cells in a microfluidic platform by using a brain cell co-culture technique so as to simulate a similar environment to the human brain in vitro. The NVU-on-a-chip includes an extracellular matrix (ECM) simulation material (70) in a gel state; and at least one channel (75) which passes through the ECM simulation material (70) and perfuses a culture medium, in which the ECM simulation material (70) contains a plurality of types of human brain cells on an outer side of the channel (75), a brain microvessel endothelial cell lining (91) is formed on an inner side of the channel (75), and the plurality of types of human brain cells and the brain microvessel endothelial cell lining (91) contact each other through the channel (75) to simulate a blood brain barrier (BBB) of a human brain and a neurovascular unit (NVU) of the human brain including the BBB.

公开(公告)号：US11021586B2

公开(公告)日：2021-06-01

申请号：US15438215

申请日：2017-02-21

Applicant: Korea Institute of Science and Technology

Inventor： Nakwon Choi ,  Sohyun Kim ,  Sohyeon Jeong ,  Eui Sung Yoon ,  Changjoon Justin Lee ,  Il-Joo Cho

IPC: B29C43/18 ,  C08J7/043 ,  C08J7/04 ,  C12M3/00 ,  C12M1/12 ,  C12N5/00 ,  C12N5/09

Abstract: The present disclosure relates to an apparatus and a method for manufacturing a channel-coupled scaffold. The present disclosure provides a method for manufacturing a channel-coupled scaffold, which includes: (1) a step of compressing a first elastic substrate which includes a groove on the surface of the substrate to close the groove; (2) a step of loading a scaffold composition onto the closed groove; and (3) a step of restoring the elastic substrate. The present disclosure also provides an apparatus for manufacturing a channel-coupled scaffold, which includes: a first elastic substrate which includes a groove on the surface of the substrate and onto which a scaffold composition is loaded: and a compression module which compresses the width of the groove of the elastic substrate to close it. The apparatus or method may accumulate a microchannel controlling local mass transfer, and align a collagen fiber in the scaffold at the same time.

公开(公告)号：US10648024B2

公开(公告)日：2020-05-12

申请号：US15032541

申请日：2014-10-28

Applicant: Korea Institute of Science and Technology

Inventor： Sang Kyung Kim ,  Nakwon Choi ,  Dong Jin Lee ,  Seungwon Jung

IPC: B01L3/00 ,  C12Q1/686

Abstract: A porous structure according to the present invention has a polymerase chain reaction (PCT) primer inside pores thereof, and hence, even an inner portion thereof can be used unlike general structures of which only surfaces are used for amplification and detection, thereby maximizing reactivity. In addition, the differentiating of the kinds of primers contained in respective structures leads to detection of several kinds of target nucleic acids at the same and real-time analysis thereof at the same time, and thus is useful for multiplex real-time PCR.