公开(公告)号：US10006920B2

公开(公告)日：2018-06-26

申请号：US14787146

申请日：2014-04-25

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Young Soo Kim ,  Dong Jin Kim ,  Hye Yun Kim ,  Soo Min Cho ,  Tae Song Kim ,  Hyunjin Kim ,  Sejin Lee ,  Kyoseon Hwang ,  Jee Hoon Roh ,  Jae-Young Koh

IPC: G01N33/68 ,  A61K38/17 ,  C07K14/47

CPC classification number: G01N33/6893 ,  A61K38/1716 ,  C07K14/4711 ,  G01N33/6827 ,  G01N33/6896 ,  G01N2333/4709 ,  G01N2800/2821

Abstract: The present disclosure relates to a diagnostic kit capable of accurately diagnosing diseases or disorders related with abnormal aggregation or misfolding of proteins, including disorders or diseases caused by aggregation of β-amyloid such as Alzheimer's disease as well as disorders or diseases caused by aggregation of other proteins, based on concentration analysis of the aggregated proteins before and after dissociation.

公开(公告)号：US20170131233A1

公开(公告)日：2017-05-11

申请号：US14994389

申请日：2016-01-13

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong-Sik Shin ,  Jae-hoon Ji ,  Kyoseon Hwang ,  Tae Song Kim

IPC: G01N27/416 ,  G01N33/68 ,  G01N27/327

CPC classification number: G01N27/4166 ,  G01N27/3275 ,  G01N27/745 ,  G01N33/5438

Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.

公开(公告)号：US10648988B2

公开(公告)日：2020-05-12

申请号：US15878351

申请日：2018-01-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong-Sik Shin ,  Jae-hoon Ji ,  Youhee Heo ,  Kyoseon Hwang ,  Myung-Sic Chae

IPC: G01N33/53 ,  G01N25/18 ,  G01N24/00 ,  G01N27/00 ,  G01N33/68 ,  A61B5/145 ,  A61B5/1468 ,  A61B5/00 ,  A61B5/1486 ,  C12Q1/6883

Abstract: A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.

公开(公告)号：US10281419B2

公开(公告)日：2019-05-07

申请号：US15105740

申请日：2016-03-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kyoseon Hwang ,  Nakwon Choi ,  Jinsik Kim ,  Hye Jin Kim ,  Yong Kyoung Yoo ,  Sang Yun Yeom ,  Woongsun Choi

IPC: G01N27/12 ,  G01N33/53 ,  G01N33/483 ,  G01N33/487 ,  G01N33/543

Abstract: A hydrogel-based interdigitated microelectrode biosensor is disclosed. The hydrogel-based interdigitated microelectrode biosensor includes: a first interdigitated microelectrode having a plurality of first protrusion electrodes arranged in a comb-like shape on a substrate; a second interdigitated microelectrode facing the first interdigitated microelectrode and having a plurality of second protrusion electrodes arranged in a comb-like shape on the substrate, the second protrusion electrodes being arranged alternately with the first protrusion electrodes of the first interdigitated microelectrode; and a hydrogel filled in a space between the first and second interdigitated microelectrodes arranged alternately with each other. The hydrogel is provided between the interdigitated microelectrodes such that the presence and concentration of a biological substance, such as a protein, are detected by measuring the impedance between the interdigitated microelectrodes.

公开(公告)号：US11313831B2

公开(公告)日：2022-04-26

申请号：US16077074

申请日：2016-11-09

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kyoseon Hwang ,  Jinsik Kim ,  Hye Jin Kim ,  Yong Kyoung Yoo ,  Youngsoo Kim

IPC: B03C5/00 ,  G01N33/543 ,  G01N27/447 ,  B03C5/02

Abstract: An interdigitated electrode biosensor includes an insulating layer configured to fully cover a sensor forming region of a substrate, a first interdigitated microelectrode configured such that a plurality of first protruding electrodes is arranged in a shape of a comb on the substrate, a second interdigitated microelectrode configured such that a plurality of second protruding electrodes is arranged in a shape of a comb and each interdigitates with the plurality of first protruding electrodes, and a plurality of receptors that is immobilized in a space between the first interdigitated microelectrode and the second interdigitated microelectrode and reacts specifically to target biomaterials. Different voltages are uniformly or nonuniformly applied to the first interdigitated microelectrode and the second interdigitated microelectrode to generate a dielectrophoretic force by a nonuniform electric field, improving the sensor by increasing the probability of specific reaction with the target biomaterials using the concentration effect through dielectrophoresis.

公开(公告)号：US11313828B2

公开(公告)日：2022-04-26

申请号：US14994389

申请日：2016-01-13

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ji Yoon Kang ,  Kyeong-Sik Shin ,  Jae-hoon Ji ,  Kyoseon Hwang ,  Tae Song Kim

IPC: G01N27/416 ,  G01N27/327 ,  G01N33/543

Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.

公开(公告)号：US09753027B2

公开(公告)日：2017-09-05

申请号：US14575129

申请日：2014-12-18

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kyoseon Hwang ,  Tae Song Kim ,  Rhokyun Kwak ,  Won Woo Cho ,  Myung Sic Chae

IPC: G01N29/00 ,  G01H11/08 ,  H01L41/08 ,  G01N33/53 ,  G01N33/543 ,  G01N29/02 ,  G01N29/036

CPC classification number: G01N33/5302 ,  G01N29/022 ,  G01N29/036 ,  G01N33/54373 ,  G01N33/5438 ,  G01N2291/0427

Abstract: The present disclosure relates to a cantilever sensor, and a biosensor having the same, wherein the cantilever sensor including a slit formed on a flat board and a cantilever formed by the slit, a first electrode formed on the cantilever, and a second electrode formed on the flat board countered to the first electrode about the slit, wherein the electrodes are formed on the cantilever sensor having the slit, whereby sensing can be conducted by an electric method, through which the sensor can be effectively miniaturized.