公开(公告)号：US20160097096A1

公开(公告)日：2016-04-07

申请号：US14806514

申请日：2015-07-22

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Jeong Ki MIN ,  Jongjin PARK ,  Kwang-Hee BAE ,  Sang Chul LEE ,  Yee Sook CHO

IPC: C12Q1/68 ,  C07K16/28 ,  G01N33/569

CPC classification number: C12Q1/6881 ,  C07K16/28 ,  C12Q2600/158 ,  G01N33/56966 ,  G01N2333/705 ,  G01N2400/00

Abstract: The present invention relates to a composition for detecting the undifferentiated human pluripotent stem cells comprising an agent useful for measuring the level of Desmoglein 2 (Dsg 2) mRNA or the protein thereof, a kit for detecting the undifferentiated human pluripotent stem cells comprising the said composition, a method for detecting the undifferentiated human pluripotent stem cells containing the step of measuring the level of Desmoglein 2 mRNA or the protein thereof, a method for evaluating the differentiation of human pluripotent stem cells and thereafter for separating the undifferentiated human pluripotent stem cells, a method for reducing the undifferentiated status of human pluripotent stem cells by inhibiting the expression or activation of Desmoglein 2, and a monoclonal antibody binding specifically to human Desmoglein 2.

Abstract translation: 本发明涉及一种用于检测未分化的人多能干细胞的组合物，其包含可用于测定脱骨髓糖蛋白2（Dsg2）mRNA或其蛋白质的水平的试剂，用于检测未分化的人多能干细胞的试剂盒，所述试剂盒包含所述组合物 用于检测未分化的人多能干细胞的方法，所述方法包括测定脱骨髓蛋白素2 mRNA或其蛋白质的水平的步骤，评估人多能干细胞分化的方法，然后分离未分化的人多能干细胞， 通过抑制脱骨髓素蛋白2的表达或活化来减少人多能干细胞未分化状态的方法，以及特异性结合人骨髓蛋白2的单克隆抗体。