公开(公告)号：US20130059349A1

公开(公告)日：2013-03-07

申请号：US13696193

申请日：2011-05-06

申请人： Kozo Murao ,  Makoto Kano ,  Yuji Hirata

发明人： Kozo Murao ,  Makoto Kano ,  Yuji Hirata

IPC分类号： C12P13/02 ,  C12M1/38

CPC分类号： C12P13/02 ,  C12M21/18 ,  C12M41/22 ,  C12Y402/01084

摘要： The present invention provides a more efficient method for producing acrylamide from acrylonitrile by the action of a microbially-derived enzyme, nitrile hydratase. More specifically, the present invention provides a method for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase, which comprises the step of keeping acrylonitrile while cooling to less than 30° C. Moreover, the present invention also provides an apparatus for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase.

摘要翻译： 本发明提供了通过微生物来源的酶腈水合酶的作用从丙烯腈生产丙烯酰胺的更有效的方法。 更具体地，本发明提供了一种使用具有腈水合酶的生物催化剂从丙烯腈制备丙烯酰胺的方法，其包括在冷却至低于30℃时保持丙烯腈的步骤。此外，本发明还提供了一种从 丙烯腈使用具有腈水合酶的生物催化剂。

公开(公告)号：US5580765A

公开(公告)日：1996-12-03

申请号：US191164

申请日：1994-02-03

申请人： Yoshihiro Hashimoto ,  Takakazu Endo ,  Koji Tamura ,  Yuji Hirata

发明人： Yoshihiro Hashimoto ,  Takakazu Endo ,  Koji Tamura ,  Yuji Hirata

IPC分类号： C12P7/42 ,  C12P41/00 ,  C12R1/01

CPC分类号： C12P41/006 ,  C12P7/42 ,  Y10S435/822

摘要： A biological process for predominantly producing an optically active .alpha.-hydroxycarboxylic acid having a phenyl group directly from a racemic .alpha.-hydroxynitrile or a mixture of an aldehyde corresponding to the nitrile and prussic acid as a substrate is disclosed, comprising reacting a microorganism belonging to the genus Gordona with the substrate in a neutral to basic aqueous medium. A desired optically active .alpha.-hydroxycarboxylic acid having a phenyl group can be obtained quantitatively at a high optical purity.

摘要翻译： 公开了一种用于主要生产具有直接来自外消旋α-羟基腈的苯基的光学活性α-羟基羧酸或对应于腈和氢醌作为底物的醛的混合物的生物方法，其包括将属于 Gordona属与中性至碱性水性介质中的底物。 可以以高光学纯度定量获得具有苯基的所需光学活性α-羟基羧酸。

公开(公告)号：US5714357A

公开(公告)日：1998-02-03

申请号：US764295

申请日：1996-12-12

申请人： Yoshihiro Hashimoto ,  Takakazu Endo ,  Koji Tamura ,  Yuji Hirata ,  Etsuko Kobayashi

发明人： Yoshihiro Hashimoto ,  Takakazu Endo ,  Koji Tamura ,  Yuji Hirata ,  Etsuko Kobayashi

IPC分类号： C12P7/42 ,  C12P41/00 ,  C12R1/01 ,  C12R1/05 ,  C12R1/13 ,  C12R1/38

CPC分类号： C12P7/42 ,  C12P41/006

摘要： A biological process for predominantly producing an optically active .alpha.-hydroxycarboxylic acid having a phenyl group directly from a racemic .alpha.-hydroxynitrile or a mixture of an aldehyde corresponding to the nitrile and prussic acid as a substrate is disclosed, comprising reacting a microorganism belonging to the genus Rhodococcus, Alcaligenes, Brevibacterium or Pseudomonas with the substrate in a neutral to basic aqueous medium. A desired optically active .alpha.-hydroxycarboxylic acid having a phenyl group can be obtained quantitatively at a high optical purity.

摘要翻译： 公开了一种用于主要生产具有直接来自外消旋α-羟基腈的苯基的光学活性α-羟基羧酸或对应于腈和氢醌作为底物的醛的混合物的生物方法，其包括将属于 属于红球菌属，产碱杆菌属，短杆菌属或假单胞菌属，在中性至碱性水性介质中具有底物。 可以以高光学纯度定量获得具有苯基的所需光学活性α-羟基羧酸。

公开(公告)号：US5705382A

公开(公告)日：1998-01-06

申请号：US542291

申请日：1995-10-12

申请人： Takakazu Endo ,  Toshiaki Doi ,  Koji Tamura ,  Yuji Hirata ,  Kouzo Murao

发明人： Takakazu Endo ,  Toshiaki Doi ,  Koji Tamura ,  Yuji Hirata ,  Kouzo Murao

IPC分类号： C12N11/00 ,  C12N1/04 ,  C12N11/14 ,  C12R1/01 ,  C12N1/12 ,  C12N9/96 ,  C12N11/04

CPC分类号： C12N1/04 ,  C12N11/14

摘要： Cells having enzyme activity, and the enzyme activity thereof, are preserved for a prolonged period of time, as a suspension of microbial cells or as a suspension of immobilized cells in particles, in an aqueous medium that is a neutral or weakly basic aqueous solution of inorganic salts, having a molarity ranging from 100 mM to the saturation concentration of the inorganic salts. Preferably, the microbial cells are cells containing the enzyme, nitrile hydratase or nitrilase, such as Gordona terrae or Rhodococcus rhodochrous, and the inorganic salts are phosphates, borates, sulfates, sulfites or hydrochlorides. The present invention provides an industrially useful method for preserving a large quantity of cells or immobilized cells in particles having nitrile hydratase or nitrilase enzyme activity for a prolonged period of time (e.g., 300 days) without cell lysis or enzyme deterioration even at room temperature. The present invention also renders possible a sharp reduction in labor and cooling cost, which are necessary in the conventional preservation process.

摘要翻译： 具有酶活性的细胞及其酶活性在水性培养基中作为微生物细胞的悬浮液或作为固定化细胞的悬浮液在水性培养基中保持较长时间，其为中性或弱碱性水溶液 无机盐，其摩尔浓度范围为100mM至无机盐的饱和浓度。 优选地，微生物细胞是含有酶，腈水合酶或腈水解酶的细胞，例如戈多纳土壤或玫瑰色红玫瑰色，无机盐是磷酸盐，硼酸盐，硫酸盐，亚硫酸盐或盐酸盐。 本发明提供一种工业上有用的方法，即使在室温下，即使在室温下，也不会在细胞裂解或酶劣化的情况下长时间（例如，300天），在具有腈水合酶或腈水解酶活性的颗粒中保存大量细胞或固定化细胞。 本发明还使得在常规保存过程中必需的劳动和冷却成本急剧下降。