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    Magnetic read/write slider with micro-texture and method for forming the same
    1.
    发明申请
    Magnetic read/write slider with micro-texture and method for forming the same 有权
    具有微纹理的磁读/写滑块及其形成方法

    公开(公告)号：US20070050971A1

    公开(公告)日：2007-03-08

    申请号：US11218710

    申请日：2005-09-06

    申请人： Kunihiro Ueda ,  Hong Ma ,  Hong Fang ,  Xiao Qiao ,  Heng Qiao ,  Yong Zhu

    发明人： Kunihiro Ueda ,  Hong Ma ,  Hong Fang ,  Xiao Qiao ,  Heng Qiao ,  Yong Zhu

    IPC分类号： G11B5/127

    CPC分类号： G11B5/1871 ,  G11B5/3163 ,  G11B5/6005 ,  Y10T29/49032 ,  Y10T29/49041

    摘要： A method for forming micro-texture on an air bearing surface of a magnetic read/write slider, including: positioning sliders arranged in arrays on a tray, each slider having a pole tip facing upward; loading the tray into a processing chamber, which is then evacuated to a preset pressure; introducing a processing gas containing oxygen into the processing chamber; exposing the sliders to an etching means in the atmosphere of the processing gas to etch the surface of the slider so as to form a clear two-step structure thereon.

    摘要翻译： 一种用于在磁读/写滑块的空气轴承表面上形成微纹理的方法，包括：将排列成阵列的滑块定位在托盘上，每个滑块具有朝上的极尖; 将托盘装载到处理室中，然后将其抽真空至预设压力; 将含有氧气的处理气体引入所述处理室中; 在处理气体的气氛中将滑块暴露于蚀刻装置，以蚀刻滑块的表面，以便在其上形成清晰的两步结构。

    Genetically engineered yeast with modified signal peptidase complex
    2.
    发明授权
    Genetically engineered yeast with modified signal peptidase complex 失效
    具有修饰信号肽酶复合物的遗传工程酵母

    公开(公告)号：US06197545B1

    公开(公告)日：2001-03-06

    申请号：US09269345

    申请日：1999-03-25

    申请人： Hong Fang ,  Neil Green ,  Luc Van Kaer

    发明人： Hong Fang ,  Neil Green ,  Luc Van Kaer

    IPC分类号： C12N1581

    CPC分类号： C12N9/52 ,  C12N15/81

    摘要： The present invention provides a yeast cell comprising a nucleic acid functionally encoding a eukaryotic (e.g., mammalian) protein homologue of a subunit (Sec11p, Spc1p, Spc2p and/or Spc3p) of the yeast signal peptidase complex. The yeast cell of this invention can be, for example, of the genus Saccharomyces, Schizosaccharomyces, Pichia, Hansenula, Kluyveromyces and/or Yarrowia. Furthermore, the yeast cell can lack one or more functional subunits (Sec11p, Spc1p, Spc2p and/or Spc3p) of the yeast signal peptidase complex. The present invention further provides a method for producing a protein heterologous to a yeast cell comprising expressing, in the yeast cell of this invention, a nucleic acid functionally encoding the heterologous protein under conditions which permit the expression of the nucleic acid as a precursor protein having a signal peptide and processing of the precursor protein to a signal peptide-cleaved form of the protein.

    摘要翻译： 本发明提供了酵母细胞，其包含功能性编码酵母信号肽酶复合物的亚基（Sec11p，Spc1p，Spc2p和/或Spc3p）的真核（例如哺乳动物）蛋白质同源物的核酸。 本发明的酵母细胞可以是例如酵母属，裂殖酵母属，毕赤酵母，汉逊酵母，克鲁维酵母和/或耶氏酵母属。 此外，酵母细胞可以缺乏酵母信号肽酶复合物的一个或多个功能性亚基（Sec11p，Spc1p，Spc2p和/或Spc3p）。 本发明还提供了用于产生与酵母细胞异源的蛋白质的方法，包括在本发明的酵母细胞中表达在允许以表达核酸作为前体蛋白的条件下功能性编码异源蛋白质的核酸， 信号肽和前体蛋白加工成信号肽切割形式的蛋白质。

    Cell-based assay for identifying peptidase inhibitors
    4.
    发明申请
    Cell-based assay for identifying peptidase inhibitors 审中-公开
    用于鉴定肽酶抑制剂的基于细胞的测定

    公开(公告)号：US20050136394A1

    公开(公告)日：2005-06-23

    申请号：US10842846

    申请日：2004-05-11

    申请人： Hong Fang ,  Neil Green

    发明人： Hong Fang ,  Neil Green

    IPC分类号： C12N1/19 ,  C12N9/64 ,  C12Q1/02 ,  C12Q1/37 ,  C12Q1/00

    CPC分类号： C12N9/6489 ,  C12Q1/02 ,  C12Q1/37 ,  G01N2333/39

    摘要： The present invention provides assays for the identification of inhibitors of endopeptidase toxins. The assays utilize genetically engineered yeast cells that contain a conditionally expressed endopeptidase toxin. When conditions for expression of the toxin are met, the toxin cleaves a yeast (natural or engineered) peptide product that is required for yeast survival. If the yeast is grown in the presence of an candidate substance that is an inhibitor of the toxin, the yeast survives, thereby providing a rapid and sensitive identification of the inhibitor.

    摘要翻译： 本发明提供了鉴定内肽酶毒素抑制剂的方法。 该测定法利用含有条件表达的内肽酶毒素的遗传工程酵母细胞。 当满足毒素表达条件时，毒素切割酵母存活所需的酵母（天然或工程化）肽产物。 如果酵母在存在作为毒素抑制剂的候选物质的情况下生长，则酵母存活，从而提供抑制剂的快速和敏感的鉴定。