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1.发明申请Genome-Wide Construction of Schizosaccharomyces Pombe Heterozygous Deletion Mutants Containing Gene-Specific Barcodes by the Methods of 4-Round Serial or Block PCR, or Total Gene Synthesis Thereof 有权通过四轮序列或块PCR方法或其总基因合成方法，含有基因特异性条形码的裂殖酵母属Pombe Pombe杂合子缺失突变体的基因组构建公开(公告)号：US20110190163A1
公开(公告)日：2011-08-04
申请号：US12989192
申请日：2008-08-27
申请人： Kwang Lae Hoe , Dong Uk Kim , Mi Sun Won , Hyang Sook Yoo , Dong Sup Kim , Han Oh Park , Kyung Sook Chung , Young Joo Jang , Mi Young Nam , Sang Jo Han , Hin Jung Choi , Seung Tae Baek , Hyong Bai Kim , Kyung Sun Heo , Hye Mi Lee , Min Ho Lee , Jo Young Park
发明人： Kwang Lae Hoe , Dong Uk Kim , Mi Sun Won , Hyang Sook Yoo , Dong Sup Kim , Han Oh Park , Kyung Sook Chung , Young Joo Jang , Mi Young Nam , Sang Jo Han , Hin Jung Choi , Seung Tae Baek , Hyong Bai Kim , Kyung Sun Heo , Hye Mi Lee , Min Ho Lee , Jo Young Park
CPC分类号： C12N15/1034 , C12N1/00 , C40B30/00 , C40B40/02 , C40B50/00
摘要： A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
摘要翻译： 提供用于制备基因靶向杂合缺失粟酒裂殖酵母的方法，该方法包括用含有同源重组位点的四轮连续PCR，阻断PCR或全基因合成构建的缺失盒转化粟酒裂殖酵母。 还提供了通过该方法制备的基因靶向杂合缺失粟酒裂殖酵母突变体和基因靶向杂合缺失粟酒裂殖酵母突变体的文库。 此外，图书馆在构建用于筛选药物作用模式的方法和试剂盒方面是有用的。
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公开(公告)号：US09670480B2
公开(公告)日：2017-06-06
申请号：US12989192
申请日：2008-08-27
申请人： Kwang Lae Hoe , Dong Uk Kim , Mi Sun Won , Hyang Sook Yoo , Dong Sup Kim , Han Oh Park , Kyung Sook Chung , Young Joo Jang , Mi Young Nam , Sang Jo Han , Shin Jung Choi , Seung Tae Baek , Hyong Bai Kim , Kyung Sun Heo , Hye Mi Lee , Min Ho Lee , Jo Young Park
发明人： Kwang Lae Hoe , Dong Uk Kim , Mi Sun Won , Hyang Sook Yoo , Dong Sup Kim , Han Oh Park , Kyung Sook Chung , Young Joo Jang , Mi Young Nam , Sang Jo Han , Shin Jung Choi , Seung Tae Baek , Hyong Bai Kim , Kyung Sun Heo , Hye Mi Lee , Min Ho Lee , Jo Young Park
CPC分类号： C12N15/1034 , C12N1/00 , C40B30/00 , C40B40/02 , C40B50/00
摘要： A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
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