公开(公告)号：US20240271327A1

公开(公告)日：2024-08-15

申请号：US18643692

申请日：2024-04-23

Applicant: Denali Therapeutics Inc.

Inventor： Xiaocheng Chen ,  Mark S. Dennis ,  Mihalis Kariolis ,  Adam P. Silverman ,  Ankita Srivastava ,  Ryan J. Watts ,  Robert C. Wells ,  Joy Yu Zuchero

IPC: C07K16/28 ,  C07K16/00 ,  C12N15/10 ,  C40B20/00 ,  C40B30/04 ,  C40B50/00

CPC classification number: C07K16/2881 ,  C07K16/005 ,  C12N15/1058 ,  C40B20/00 ,  C40B30/04 ,  C40B50/00 ,  C07K2317/35 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/55 ,  C12N2320/10

Abstract: Provided herein are polypeptides that bind to a transferrin receptor, methods of generating such polypeptides, and methods of using the polypeptides to target a composition to a transferrin receptor-expressing cell.

公开(公告)号：US20240141058A1

公开(公告)日：2024-05-02

申请号：US18408477

申请日：2024-01-09

Applicant: Denali Therapeutics Inc.

Inventor： Xiaocheng Chen ,  Mark S. Dennis ,  Mihalis Kariolis ,  Adam P. Silverman ,  Ankita Srivastava ,  Ryan J. Watts ,  Robert C. Wells ,  Joy Yu Zuchero

IPC: C07K16/28 ,  C07K16/00 ,  C12N15/10 ,  C40B20/00 ,  C40B30/04 ,  C40B50/00

CPC classification number: C07K16/2881 ,  C07K16/005 ,  C12N15/1058 ,  C40B20/00 ,  C40B30/04 ,  C40B50/00 ,  C07K2317/35 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/55 ,  C12N2320/10

Abstract: Provided herein are polypeptides that bind to a transferrin receptor, methods of generating such polypeptides, and methods of using the polypeptides to target a composition to a transferrin receptor-expressing cell.

公开(公告)号：US20180265912A1

公开(公告)日：2018-09-20

申请号：US15853055

申请日：2017-12-22

Applicant: Rutgers, The State University of New Jersey

Inventor： Bin Tian ,  Dinghai Zheng

IPC: C12Q1/6806 ,  C07H21/02 ,  C40B50/00 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12N9/22 ,  C12N9/00 ,  C07H19/06

CPC classification number: C12Q1/6806 ,  C07H19/06 ,  C07H21/02 ,  C12N9/22 ,  C12N9/93 ,  C12N2310/11 ,  C12N2310/3181 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2525/173 ,  C12Q2600/158 ,  C12Q2600/166 ,  C40B50/00 ,  G16B30/00 ,  G16B30/10 ,  C12Q2521/327 ,  C12Q2525/191

Abstract: The present invention relates to modified 3′ region extraction and deep sequencing of polyadenylated RNA to identify a poly(A) site in a reference, as well as to calculate poly(A) tail length.

公开(公告)号：US20160023179A1

公开(公告)日：2016-01-28

申请号：US14875514

申请日：2015-10-05

Applicant: ARRAYOMICS, INC.

Inventor： Christopher D. Herold ,  David Rothwarf ,  Bao Nguyen

IPC: B01J19/00 ,  G06K19/06

CPC classification number: B01J19/0046 ,  B01J2219/00497 ,  B01J2219/00549 ,  B01J2219/00563 ,  B01J2219/00585 ,  B01J2219/00655 ,  B01J2219/00709 ,  C40B40/00 ,  C40B50/00 ,  G01N33/54326 ,  G01N33/54366 ,  G06K19/06187

Abstract: Microdevices containing a predetermined preferential axis of magnetization are disposed in an array having discreet regions. Under influence of a magnetic field, the microdevices can have at least twelve discrete orientations, and can advantageously be flipped upside down in place. Microdevices can be coded in a manner that supports a coding space of at least 102, 103, 106 or even 1010 or more choices, and can include one or more chemically reactive sites. The regions can be defined by long and short bars, in which microdevices span gaps between the longer bars, and the shorter bars measure less than 60% of such gaps. Preferred embodiments are also provided to produce microfabricated microdevices for magnetic assembly-based arraying.

Abstract translation: 包含预定的磁化优选轴的微器件设置在具有谨慎区域的阵列中。 在磁场的影响下，微器件可以具有至少十二个离散取向，并且可以有利地在适当位置上翻翻。 可以以支持至少102,103,106甚至1010或更多选择的编码空间的方式对微器件进行编码，并且可以包括一个或多个化学反应位点。 这些区域可以由长条和短条定义，其中微型设备跨越长条之间的间隙，较短的条测量小于这种间隙的60％。 还提供了优选实施例以产生用于基于磁性组件的排列的微加工微型器件。

公开(公告)号：US20130045892A1

公开(公告)日：2013-02-21

申请号：US13695423

申请日：2011-04-27

Applicant: Johannes Grillari ,  Regina Grillari ,  Otto Kanzler

Inventor： Johannes Grillari ,  Regina Grillari ,  Otto Kanzler

IPC: C40B30/06 ,  C40B50/06 ,  C40B40/02

CPC classification number: G01N33/5044 ,  C40B50/00 ,  G01N33/5014

Abstract: Panels of mammalian cell samples, kits and methods of use thereof. The panels comprise samples or sets of samples of immortalized primary cells, obtained from different tissues and organs of healthy and/or diseased donors. The cell panels are useful in assays for testing effects of drug candidates, e.g. for activity and toxicity.

Abstract translation: 哺乳动物细胞样品组，试剂盒及其使用方法。 该组包括从健康和/或患病供体的不同组织和器官获得的永生化原代细胞样品或组的样品。 细胞板可用于药物候选物的测试效果的测定，例如 用于活动和毒性。

公开(公告)号：US20080176765A1

公开(公告)日：2008-07-24

申请号：US12018439

申请日：2008-01-23

Applicant: David Rothwarf

Inventor： David Rothwarf

IPC: C40B40/06 ,  C40B50/16 ,  C40B40/00 ,  B07C5/344 ,  C40B40/14 ,  C40B40/10

CPC classification number: B01J19/0046 ,  B01J2219/00497 ,  B01J2219/00549 ,  B01J2219/00563 ,  B01J2219/00585 ,  B01J2219/00655 ,  B01J2219/00709 ,  C40B40/00 ,  C40B50/00 ,  G01N33/54326 ,  G01N33/54366 ,  G06K19/06187

Abstract: Particles or other microdevices are disposed in an array having discreet regions (e.g. magnetic bars), oriented within a magnetic field, and then sorted through application of a removing force under conditions that remove a proper subset of the microdevices from the array as a function of differing orientations of the microdevices. Methods are also contemplated for using magnetic patterns to sort collections of microdevices by magnetic complementarity. Preferred methods use a capture and release process to sort microdevices (microdevices), and unlike conventional sorters, do not require high particle flow rates. Also contemplated are microdevice libraries in which microdevices have mutually distinct magnetic codes, and a region with a mutually distinct polymeric or other chemical moiety.

Abstract translation: 颗粒或其他微型设备被布置成具有定向在磁场内的离散区域（例如磁棒）的阵列中，然后在将阵列中的微型设备的适当子集从数组中移除的条件下通过施加去除力进行排序，作为 微器件的不同取向。 也考虑使用磁图案通过磁互补来分类微器件的集合的方法。 优选的方法使用捕获和释放过程来排序微型设备（微型设备），并且与传统的分选机不同，不需要高的颗粒流速。 还考虑了微器件库，其中微器件具有相互不同的磁性代码，以及具有相互不同的聚合物或其它化学部分的区域。

公开(公告)号：US20080092968A1

公开(公告)日：2008-04-24

申请号：US11958354

申请日：2007-12-17

Applicant: Roger WILLIAMS ,  Tarlochan Singh ,  N. Mansour ,  Lawrence Lee ,  Michael Forbush

Inventor： Roger WILLIAMS ,  Tarlochan Singh ,  N. Mansour ,  Lawrence Lee ,  Michael Forbush

IPC: F15C1/02

CPC classification number: B01L3/0268 ,  B01J19/0046 ,  B01J2219/00362 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00725 ,  B01L2300/0819 ,  B01L2400/0433 ,  B01L2400/0436 ,  B05B17/0615 ,  C40B30/04 ,  C40B40/02 ,  C40B40/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/00 ,  C40B60/14 ,  F04F7/00 ,  G01N21/11 ,  Y10T137/206 ,  Y10T137/2196 ,  Y10T436/2575

Abstract: Acoustic waves are used to transfer small amounts of fluid in a non-contact manner. Acoustic waves are propagated through a pool of a source fluid in such a manner that causes the ejection of a single micro-droplet from the surface of the pool. The droplet is ejected towards a target with sufficient force to provide for contact of the droplet with the target. Because the fluid is not contacted by any fluid transfer device such as a pipette, the opportunities for contamination are minimized. Methods may be employed to transfer fluids from an array of source sites to an array of target sites, thereby enabling the precise automation of a wide variety of procedures including screening and synthesis procedures commonly used in biotechnology.

Abstract translation: 声波用于以非接触方式转移少量流体。 声波以这样的方式传播通过源流体池，使得从池的表面喷射单个微滴。 液滴以足够的力朝向目标喷射以提供液滴与靶的接触。 由于流体不被诸如移液管之类的任何流体转移装置接触，污染的机会被最小化。 可以采用方法将流体从源位置阵列转移到靶位点阵列，从而能够精确自动化各种程序，包括通常用于生物技术的筛选和合成过程。

公开(公告)号：US20030211632A1

公开(公告)日：2003-11-13

申请号：US10444914

申请日：2003-05-22

Inventor： Roger O. Williams ,  Tarlochan Singh ,  N. Nicolas Mansour ,  Lawrence Lee JR. ,  Michael J. Forbush

IPC: G01N001/10

CPC classification number: B01L3/0268 ,  B01J19/0046 ,  B01J2219/00362 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00725 ,  B01L2300/0819 ,  B01L2400/0433 ,  B01L2400/0436 ,  B05B17/0615 ,  C40B30/04 ,  C40B40/02 ,  C40B40/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/00 ,  C40B60/14 ,  F04F7/00 ,  G01N21/11 ,  Y10T137/206 ,  Y10T137/2196 ,  Y10T436/2575

Abstract: Acoustic waves are used to transfer small amounts of fluid in a non-contact manner. Acoustic waves are propagated through a pool of a source fluid in such a manner that causes the ejection of a single micro-droplet from the surface of the pool. The droplet is ejected towards a target with sufficient force to provide for contact of the droplet with the target. Because the fluid is not contacted by any fluid transfer device such as a pipette, the opportunities for contamination are minimized. Methods may be employed to transfer fluids from an array of source sites to an array of target sites, thereby enabling the precise automation of a wide variety of procedures including screening and synthesis procedures commonly used in biotechnology.

公开(公告)号：US20020094582A1

公开(公告)日：2002-07-18

申请号：US09735709

申请日：2000-12-12

Inventor： Roger O. Williams ,  Tarlochan Singh ,  N. Nicolas Mansour ,  Lawrence Lee JR. ,  Michael J. Forbush

IPC: B01L003/00

CPC classification number: B01L3/0268 ,  B01J19/0046 ,  B01J2219/00362 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00725 ,  B01L2300/0819 ,  B01L2400/0433 ,  B01L2400/0436 ,  B05B17/0615 ,  C40B30/04 ,  C40B40/02 ,  C40B40/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/00 ,  C40B60/14 ,  F04F7/00 ,  G01N21/11 ,  Y10T137/206 ,  Y10T137/2196 ,  Y10T436/2575

Abstract: Invention methods employ the use of acoustic waves to transfer small amounts of fluid in a non-contact manner. In invention methods, acoustic waves are propagated through a separated pool of a source fluid in such a manner that causes the ejection of a single micro-droplet from the surface of the pool. The droplet is ejected towards a target with sufficient force to provide for contact of the droplet with the target. Because the fluid is not contacted by any fluid transfer device such as a pipette, the opportunities for contamination are minimized. Invention methods may be employed to transfer fluids from an array of source sites to an array of target sites, thereby enabling the precision automation of a wide variety of procedures including screening, and synthesis procedures commonly used in biotechnology.

Abstract translation: 本发明的方法采用声波以非接触方式传送少量流体。 在本发明的方法中，声波以这样的方式传播通过源流体的分离池，使得从池的表面喷出单个微滴。 液滴以足够的力朝向目标喷射以提供液滴与靶的接触。 由于流体不被诸如移液管之类的任何流体转移装置接触，污染的机会被最小化。 可以采用本发明的方法将流体从源位置阵列转移到目标位点阵列，从而能够精确自动化多种程序，包括生物技术中通常使用的筛选和合成方法。

公开(公告)号：US20170066844A1

公开(公告)日：2017-03-09

申请号：US15130843

申请日：2016-04-15

Applicant: Distributed Bio, Inc.

Inventor： Jacob GLANVILLE

IPC: C07K16/46 ,  C40B30/02 ,  G06F19/16

CPC classification number: C07K16/464 ,  C07K16/465 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/567 ,  C40B30/02 ,  C40B50/00 ,  G06F19/16

Abstract: The present invention relates to a method for producing a population of nucleic acids encoding at least one protein comprising at least one immunoglobulin variable domain having a non-human-derived CDR3 amino acid sequence embedded in essentially human framework sequences, as well as to a population of nucleic acids and a population of proteins relates thereto and uses thereof.

Abstract translation: 本发明涉及编码至少一种蛋白质的核酸群体的方法，所述蛋白质包含至少一个免疫球蛋白可变结构域，其具有嵌入基本上人框架序列中的非人源性CDR3氨基酸序列，以及群体 的核酸和一组蛋白质涉及其及其用途。