公开(公告)号：US20180327719A1

公开(公告)日：2018-11-15

申请号：US15760523

申请日：2016-09-16

Applicant: Kyoto University

Inventor： Kenji OSAFUNE ,  Taro TOYODA ,  Azuma KIMURA

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N5/10 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2506/03

Abstract: Provided is a method for generating pancreatic bud cells, comprising the step of culturing PDX1+/NKX6.1− cells in a medium comprising KGF, EGY, a DMP inhibitor and an Akt inhibitor. The cells may be cultured under adherent culture conditions. PDX1+/NKX6.1− cells may be induced from pluripotent stem cells in vitro. The method of this application makes it possible to generate pancreatic bud cells from pluripotent stem cells.

公开(公告)号：US20210254014A1

公开(公告)日：2021-08-19

申请号：US17049218

申请日：2019-04-22

Applicant: Kyoto University ,  Takeda Pharmaceutical Company Limited

Inventor： Noriko YAMAZOE ,  Hideyuki HIYOSHI ,  Taisuke MOCHIDA ,  Ryo ITO ,  Taro TOYODA ,  Azuma KIMURA

IPC: C12N5/071 ,  A61K35/39

Abstract: The present invention relates to a method for producing an insulin-producing cell population or a pancreatic β cell population having the reduced number of Ki67-positive cells, comprising treating a population of endocrine progenitor cells or cells at a later stage of differentiation with an FGFR1 inhibitor.