公开(公告)号：US20240400993A1

公开(公告)日：2024-12-05

申请号：US18695720

申请日：2022-09-26

Applicant: Kyoto University ,  TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Shin KANEKO ,  Shoichi IRIGUCHI ,  Yoshiaki KASSAI ,  Keiko SEKIYA ,  Atsushi MATSUDA ,  Takayuki SATO

IPC: C12N5/0783 ,  A61K35/17

Abstract: Disclosed is a method for producing regulatory T cells, the method comprising: (1) differentiating cells that can differentiate into regulatory T cells, into which an expression construct is introduced, into regulatory T cells, the expression construct comprising: (a) conserved non-coding sequence (CNS) 1, CNS2, and CNS3 of Foxp3 gene; (b) a promoter; and (c) a nucleic acid encoding FOXP3. Also disclosed are regulatory T cells obtained by the method, and a pharmaceutical composition comprising the regulatory T cells.