公开(公告)号：US20180299456A1

公开(公告)日：2018-10-18

申请号：US16017824

申请日：2018-06-25

Applicant: Life Technologies Corporation

Inventor： Alison Sparks ,  Zhixian Wang ,  Melissa Gee

IPC: G01N33/58 ,  C07D321/00 ,  C12Q1/42 ,  C12Q1/34 ,  C07H15/203 ,  C12Q1/6816 ,  C07F9/655 ,  C07F9/6541 ,  C07F9/40 ,  C07F9/12 ,  C07F9/6558

Abstract: Methods for generating a chemiluminescent enzyme substrate in situ, in aqueous or other assay conditions. Also disclosed are methods to use the substrates to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Kits relating to these methods are also disclosed.

公开(公告)号：US20140186831A1

公开(公告)日：2014-07-03

申请号：US14070138

申请日：2013-11-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brooks Edwards ,  Timothy Geiser ,  Steven Menchen ,  Alison Sparks ,  John Voyta

IPC: C12Q1/68

CPC classification number: C12Q1/6823 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  G01N33/54393 ,  Y10S436/805 ,  Y10S436/823

Abstract: Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.

Abstract translation: 提供了化学发光测定的固体支持物。 固体支持物包括共价或物理连接到支持物表面上的多个探针和结合到表面或载体的大部分中的化学发光增强部分。 固体支持物可以是包含邻近阳离子微凝胶层的上探针结合层（例如，吖内酯聚合物层或多孔功能性聚酰胺层）的多层载体。 吖内酯官能聚合物可以是与乙二胺交联的二甲基丙烯酰胺和乙烯基内酯的共聚物。 阳离子微凝胶层可以是交联的含季铵盐的聚合物。 还提供了使用固体支持物进行化学发光测定的方法和试剂盒。 试剂盒包括二氧杂环丁烷底物，生物聚合物探针 - 酶复合物和固体支持物。 固体支持物可以是与阳离子微凝胶层相邻的吖内酯官能聚合物层; 与阳离子微凝胶层相邻的多孔聚酰胺官能层; 或季铵化的吖内酯官能聚合物层。