公开(公告)号：US11505827B2

公开(公告)日：2022-11-22

申请号：US16351456

申请日：2019-03-12

Applicant: Roche Sequencing Solutions, Inc. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Geiser ,  Randall Davis ,  Roger Chen

IPC: C12M1/34 ,  C12Q1/6874 ,  C12Q1/6869 ,  B82Y15/00

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

公开(公告)号：US20140186831A1

公开(公告)日：2014-07-03

申请号：US14070138

申请日：2013-11-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brooks Edwards ,  Timothy Geiser ,  Steven Menchen ,  Alison Sparks ,  John Voyta

IPC: C12Q1/68

CPC classification number: C12Q1/6823 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  G01N33/54393 ,  Y10S436/805 ,  Y10S436/823

Abstract: Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.

Abstract translation: 提供了化学发光测定的固体支持物。 固体支持物包括共价或物理连接到支持物表面上的多个探针和结合到表面或载体的大部分中的化学发光增强部分。 固体支持物可以是包含邻近阳离子微凝胶层的上探针结合层（例如，吖内酯聚合物层或多孔功能性聚酰胺层）的多层载体。 吖内酯官能聚合物可以是与乙二胺交联的二甲基丙烯酰胺和乙烯基内酯的共聚物。 阳离子微凝胶层可以是交联的含季铵盐的聚合物。 还提供了使用固体支持物进行化学发光测定的方法和试剂盒。 试剂盒包括二氧杂环丁烷底物，生物聚合物探针 - 酶复合物和固体支持物。 固体支持物可以是与阳离子微凝胶层相邻的吖内酯官能聚合物层; 与阳离子微凝胶层相邻的多孔聚酰胺官能层; 或季铵化的吖内酯官能聚合物层。

公开(公告)号：US10260093B2

公开(公告)日：2019-04-16

申请号：US14355203

申请日：2012-11-01

Applicant: GENIA TECHNOLOGIES, INC. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Geiser ,  Randall Davis ,  Roger Chen

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6874 ,  C12Q1/6869 ,  B82Y15/00

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

公开(公告)号：US20140303032A1

公开(公告)日：2014-10-09

申请号：US14355203

申请日：2012-11-01

Applicant: GENIA TECHNOLOGIES, INC. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Geiser ,  Randall Davis ,  Roger Chen

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B82Y15/00 ,  C12Q1/6869 ,  C12Q2525/101 ,  C12Q2537/163 ,  C12Q2565/607 ,  C12Q2565/631 ,  C12Q2525/113 ,  C12Q2525/107

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

Abstract translation: 提供了多核苷酸测序的系统和方法。 系统和方法通过纳米孔或一类蛋白质或突变蛋白优化样品（例如，多核苷酸）的控制，速度，移动和/或易位，以便积累足够的时间和电流 阻断信息以鉴定多核苷酸的单链区域中的连续核苷酸或多个核苷酸。