公开(公告)号：US20210130913A1

公开(公告)日：2021-05-06

申请号：US17099621

申请日：2020-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHANG ZHONG

IPC: C12Q1/6888 ,  C12Q1/6881 ,  C12Q1/6858

Abstract: Methods for human identification using polymorphisms in the Penta E short tandem repeat locus are significant in preventing allelic drop out. An exemplary method encompasses (a) contacting a first primer to a nucleic acid sample to be analyzed, (b) contacting a second primer to the nucleic acid sample, and (c) subjecting the nucleic acid sample, the first primer, and the second primer to an amplification reaction, and thereby forming an amplification product. The first primer, the second primer, or both the first and second primers can be labeled with a non-nucleic-acid label. Additionally, or alternatively, the amplification product can include an adenosine at position 14 from the 5′ end of SEQ ID NO:1, a thymidine at position 21 from the 5′ end of SEQ ID NO:2, or both an adenine at position 14 and a thymidine at position 21 from the 5′ end of SEQ ID NO:3.

公开(公告)号：US20170327902A1

公开(公告)日：2017-11-16

申请号：US15155440

申请日：2016-05-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHANG ZHONG

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6858 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2525/151 ,  C12Q2563/107 ,  C12Q2565/125

Abstract: Disclosed herein are methods and compositions for human identification using polymorphisms in the Penta E short tandem repeat locus. These newly disclosed polymorphisms are significant in preventing allelic drop out.