-
公开(公告)号:US20210130913A1
公开(公告)日:2021-05-06
申请号:US17099621
申请日:2020-11-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: CHANG ZHONG
IPC: C12Q1/6888 , C12Q1/6881 , C12Q1/6858
Abstract: Methods for human identification using polymorphisms in the Penta E short tandem repeat locus are significant in preventing allelic drop out. An exemplary method encompasses (a) contacting a first primer to a nucleic acid sample to be analyzed, (b) contacting a second primer to the nucleic acid sample, and (c) subjecting the nucleic acid sample, the first primer, and the second primer to an amplification reaction, and thereby forming an amplification product. The first primer, the second primer, or both the first and second primers can be labeled with a non-nucleic-acid label. Additionally, or alternatively, the amplification product can include an adenosine at position 14 from the 5′ end of SEQ ID NO:1, a thymidine at position 21 from the 5′ end of SEQ ID NO:2, or both an adenine at position 14 and a thymidine at position 21 from the 5′ end of SEQ ID NO:3.
-
公开(公告)号:US20170327902A1
公开(公告)日:2017-11-16
申请号:US15155440
申请日:2016-05-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: CHANG ZHONG
IPC: C12Q1/68
CPC classification number: C12Q1/6888 , C12Q1/6858 , C12Q1/6881 , C12Q2600/156 , C12Q2525/151 , C12Q2563/107 , C12Q2565/125
Abstract: Disclosed herein are methods and compositions for human identification using polymorphisms in the Penta E short tandem repeat locus. These newly disclosed polymorphisms are significant in preventing allelic drop out.
-