公开(公告)号：US20140004567A1

公开(公告)日：2014-01-02

申请号：US13931804

申请日：2013-06-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James HARTLEY ,  Michael Brasch ,  Gary Temple ,  Donna Fox

IPC: C12N15/64

CPC classification number: C12N15/64 ,  C12N9/00 ,  C12N15/10 ,  C12N15/66

Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

Abstract translation: 通过在体外和体内使用核酸，载体和方法，使用工程重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。