公开(公告)号：US20190001237A1

公开(公告)日：2019-01-03

申请号：US16067509

申请日：2016-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott P. Collins ,  Federico Carlos Rodriguez Peralta ,  Nephi D. Jones ,  Christopher D. Brau

IPC: B01D11/04 ,  B01D15/36 ,  B01D15/38

Abstract: Sample purification systems include a particle extraction assembly having a mixing compartment and a settling compartment. A biological sample is mixed with two liquid phases formulated to effectuate transfer of a biological molecule into a first phase and particulate contaminants into a second phase. The first phase includes a solubilizing salt, the second phase includes an organic molecule, and the mixture can have little or no monoatomic salt or dextran. The molecule-containing first phase can be optionally concentrated without also concentrating the particulate contaminants and introduced into a multi-stage liquid-liquid extractor, by which the biological molecule or molecular contaminants are extracted from the first phase into a third phase, thereby purifying the molecule away from contaminants. The extracted sample can be further purified through a series of processing steps. The system can be run in continuously mode to maintain sterility of the sample.

公开(公告)号：US20220339554A1

公开(公告)日：2022-10-27

申请号：US17861638

申请日：2022-07-11

Applicant: Life Technologies Corporation

Inventor： Scott P. Collins ,  Federico Carlos Rodriguez Peralta ,  Nephi D. Jones ,  Christopher D. Brau

IPC: B01D11/04 ,  B01D11/02 ,  B01D15/36 ,  B01D15/38

Abstract: Sample purification systems include a particle extraction assembly having a mixing compartment and a settling compartment. A biological sample is mixed with two liquid phases formulated to effectuate transfer of a biological molecule into a first phase and particulate contaminants into a second phase. The first phase includes a solubilizing salt, the second phase includes an organic molecule, and the mixture can have little or no monoatomic salt or dextran. The molecule-containing first phase can be optionally concentrated without also concentrating the particulate contaminants and introduced into a multi-stage liquid-liquid extractor, by which the biological molecule or molecular contaminants are extracted from the first phase into a third phase, thereby purifying the molecule away from contaminants. The extracted sample can be further purified through a series of processing steps. The system can be run in continuously mode to maintain sterility of the sample.

公开(公告)号：US11426679B2

公开(公告)日：2022-08-30

申请号：US16067509

申请日：2016-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott P. Collins ,  Federico Carlos Rodriguez Peralta ,  Nephi D. Jones ,  Christopher D. Brau

IPC: B01D11/04 ,  B01D15/36 ,  B01D15/38 ,  B01D11/02

Abstract: Sample purification systems include a particle extraction assembly having a mixing compartment and a settling compartment. A biological sample is mixed with two liquid phases formulated to effectuate transfer of a biological molecule into a first phase and particulate contaminants into a second phase. The first phase includes a solubilizing salt, the second phase includes an organic molecule, and the mixture can have little or no monoatomic salt or dextran. The molecule-containing first phase can be optionally concentrated without also concentrating the particulate contaminants and introduced into a multi-stage liquid-liquid extractor, by which the biological molecule or molecular contaminants are extracted from the first phase into a third phase, thereby purifying the molecule away from contaminants. The extracted sample can be further purified through a series of processing steps. The system can be run in continuously mode to maintain sterility of the sample.