公开(公告)号：US20150210989A1

公开(公告)日：2015-07-30

申请号：US14603256

申请日：2015-01-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey ROGERS ,  Jason Potter

IPC: C12N9/12 ,  C12N15/10 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12P19/34 ,  C12Y207/07049

Abstract: The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.

Abstract translation: 本发明提供具有所需性质的新型逆转录酶（RT），例如增加的热稳定性，增加的热反应性和/或增加的对抑制剂的抗性。 在某些实施方案中，本发明提供使用包含这种新型逆转录酶的试剂盒，组合物和/或反应混合物来产生，扩增和/或测序核酸分子（特别是cDNA分子）的方法。

公开(公告)号：US09663770B2

公开(公告)日：2017-05-30

申请号：US14603256

申请日：2015-01-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Rogers ,  Jason Potter

IPC: C12N9/12 ,  C12N15/10 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12P19/34 ,  C12Y207/07049

Abstract: The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.