公开(公告)号：US10066000B2

公开(公告)日：2018-09-04

申请号：US13886226

申请日：2013-05-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Sanjay Vasu ,  Henry Chiou ,  Jeffrey Rogers ,  Maria Isabel Cisneros ,  Jingqiu Li ,  Chao Yan Liu ,  Meredith Jones

IPC: C12P21/02 ,  C12N5/02 ,  C07K14/505 ,  C12N5/00 ,  C12N5/07 ,  C12N5/073 ,  C12N5/10 ,  C12N5/16 ,  C12N5/22

Abstract: High-Yield mammalian transient expression systems can include a cell culture media (particularly serum free, non animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

公开(公告)号：US20160299145A1

公开(公告)日：2016-10-13

申请号：US15132821

申请日：2016-04-19

Applicant: Life Technologies Corporation

Inventor： Bradley Love ,  Jeffrey Rogers ,  Joseph Beechem ,  Lilin Wang

IPC: G01N33/574 ,  G01N33/564

CPC classification number: G01N33/57434 ,  C12Y207/10001 ,  C12Y207/11001 ,  G01N33/564 ,  G01N2333/91205

Abstract: Provided herein are novel autoantibody biomarkers, and panels for detecting autoantibody biomarkers for prostate cancer, and methods and kits for detecting these biomarkers in the serum of individuals suspected of having prostate cancer.

Abstract translation: 本文提供了新颖的自身抗体生物标志物和用于检测前列腺癌的自身抗体生物标志物的小组，以及用于检测怀疑患有前列腺癌的个体的血清中的这些生物标志物的方法和试剂盒。

公开(公告)号：US09663770B2

公开(公告)日：2017-05-30

申请号：US14603256

申请日：2015-01-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Rogers ,  Jason Potter

IPC: C12N9/12 ,  C12N15/10 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12P19/34 ,  C12Y207/07049

Abstract: The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.

公开(公告)号：US20150267209A1

公开(公告)日：2015-09-24

申请号：US14437317

申请日：2013-10-22

Applicant: Life Technologies Corporation

Inventor： Jeffrey Rogers ,  Julia Fletcher ,  Kevin Lowitz

IPC: C12N15/67 ,  C12N9/38 ,  C07K2/00 ,  C12P21/00 ,  C12N15/70

CPC classification number: C12N15/67 ,  C07K2/00 ,  C07K2319/40 ,  C07K2319/60 ,  C12N9/2471 ,  C12N15/1086 ,  C12N15/63 ,  C12N15/70 ,  C12P21/00

Abstract: The present disclosure describes a novel recombinant gene expression system and a method of its use. The recombinant gene expression system is adapted to enable a user to monitor the successful expression of a protein in real time by allowing a small portion (e.g., less than 10%) of the target protein to be expressed as a fusion with a readily detectable reporter. The recombinant gene expression system further allows a user to select a clone that exhibits high, medium or low expression by providing a random ribosomal binding site having the nucleotide sequence N4R6NX, where N is A, T, G, or C, where R is A or G, and where x is an integer from 6 to 11 (SEQ ID NO: 1) upstream of the cloned gene. By selecting a clone exhibiting a desired relative level of reporter gene expression, a user can tailor the expression level of the desired protein.

Abstract translation: 本公开描述了一种新的重组基因表达系统及其使用方法。 重组基因表达系统适于使用户能够通过使目标蛋白质的一小部分（例如，小于10％）表达为与容易检测的报道分子的融合物来实时监测蛋白质的成功表达 。 重组基因表达系统还允许用户通过提供具有核苷酸序列N4R6NX的随机核糖体结合位点来选择表现出高，中或低表达的克隆，其中N是A，T，G或C，其中R是A 或G，并且其中x是克隆基因上游的6至11（SEQ ID NO：1）的整数。 通过选择表现出所需相对水平的报告基因表达的克隆，用户可以调整所需蛋白质的表达水平。

公开(公告)号：US20160264950A1

公开(公告)日：2016-09-15

申请号：US14835609

申请日：2015-08-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Rogers ,  Bianca Lam ,  Joanna Guo ,  Lushen Li

IPC: C12N9/12 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12N9/12 ,  C12N9/99 ,  C12P19/34 ,  C12Y207/07049

Abstract: The present invention provides reversibly inactivated reverse transcriptase enzymes, particularly those having increased thermostability and/or thermoreactivity and compositions, methods and kits that include such enzymes, for the reverse transcription of nucleic acid molecules. Also provided are compositions and methods for the reactivation of reversibly inactivated reverse transcriptases. More particularly, the present invention relates to compositions and methods that can increase the speed of reactivation and stabilize reactivation of chemically modified reverse transcriptase enzymes prior to, or as part of a reverse transcription reaction. As compared to existing compositions and methods, the reversibly inactivated reverse transcriptase enzymes of the present invention provide for a significant reduction in non-specific reverse transcription from template nucleic acid molecules.

Abstract translation: 本发明提供了可逆地灭活的逆转录酶，特别是那些具有增加的热稳定性和/或热反应性的组合物，包含这些酶的组合物，方法和试剂盒，用于逆转录核酸分子。 还提供了用于重新激活可逆失活的逆转录酶的组合物和方法。 更具体地说，本发明涉及可以在逆转录反应之前或作为逆转录反应的一部分期间增加再活化速度并稳定化学修饰的逆转录酶的再活化的组合物和方法。 与现有的组合物和方法相比，本发明的可逆失活的逆转录酶提供了模板核酸分子的非特异性逆转录的显着降低。