公开(公告)号：US10941439B2

公开(公告)日：2021-03-09

申请号：US16176231

申请日：2018-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US20190062807A1

公开(公告)日：2019-02-28

申请号：US16176231

申请日：2018-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US10150992B2

公开(公告)日：2018-12-11

申请号：US15202487

申请日：2016-07-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Krishnan Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.