公开(公告)号：US09901887B2

公开(公告)日：2018-02-27

申请号：US15282283

申请日：2016-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech ,  John Andrew Sheridan

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  B01F3/08 ,  C12P19/34 ,  C12M1/26 ,  B01F15/00 ,  B04B11/02 ,  B01F5/04 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  B04B5/04 ,  B04B7/02 ,  C12Q1/68

CPC classification number: B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F15/00 ,  B01F2215/0037 ,  B01L3/5021 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0409 ,  B04B5/0414 ,  B04B7/02 ,  B04B11/02 ,  B04B2007/025 ,  C12M33/10 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/32 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

公开(公告)号：US20190062807A1

公开(公告)日：2019-02-28

申请号：US16176231

申请日：2018-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US20170014784A1

公开(公告)日：2017-01-19

申请号：US15282283

申请日：2016-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan SCHULTZ ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech ,  John Andrew Sheridan

IPC: B01F3/08 ,  B01L7/00 ,  B01F13/00 ,  B04B11/02 ,  B04B7/02 ,  B04B5/04 ,  C12Q1/68 ,  B01L3/00

CPC classification number: B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F15/00 ,  B01F2215/0037 ,  B01L3/5021 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0409 ,  B04B5/0414 ,  B04B7/02 ,  B04B11/02 ,  B04B2007/025 ,  C12M33/10 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/32 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

公开(公告)号：US10941439B2

公开(公告)日：2021-03-09

申请号：US16176231

申请日：2018-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US09121047B2

公开(公告)日：2015-09-01

申请号：US13775855

申请日：2013-02-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech ,  Andrew Sheridan

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  C12M1/36 ,  C12M1/38 ,  C12P19/34 ,  C12M1/26 ,  B01F5/04 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  B04B5/04 ,  B04B7/02 ,  C12Q1/68

CPC classification number: B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F15/00 ,  B01F2215/0037 ,  B01L3/5021 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0409 ,  B04B5/0414 ,  B04B7/02 ,  B04B11/02 ,  B04B2007/025 ,  C12M33/10 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/32 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。