公开(公告)号：US20200010888A1

公开(公告)日：2020-01-09

申请号：US16355607

申请日：2019-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ming JIANG ,  Chengyong YANG ,  Eugene WANG

IPC: C12Q1/6874

Abstract: A method for improving color calls or base calls utilizes current and prior cycle multi-channel intensity data from a sequencing run to model residual cycle buildup. The model is applied to correct the multi-cycle channel intensity for the current cycle. The corrected multi-cycle channel intensity is used for color calls or base calls for the current cycle.

公开(公告)号：US20220367005A1

公开(公告)日：2022-11-17

申请号：US17805147

申请日：2022-06-02

Applicant: Life Technologies Corporation

Inventor： Ming JIANG ,  Chengyong YANG ,  Jianxin ZHANG

IPC: G16B30/10 ,  G16B30/00

Abstract: A method includes exposing template polynucleotide strands, sequencing primers, and polymerase to flows of nucleotide species; obtaining a series of measured intensity values and randomly selecting a training subset therefrom; generating series of base calls using a base caller and aligning the series of base calls to a reference genome or sequence using an aligner; determining intensity value thresholds and parameters of a linear transformation corresponding to different homopolymer lengths and nucleotide species; generating series of base calls corresponding to the series of measured intensity values using at least some of the parameters of a linear transformation; and recalibrating the series of base calls corresponding to the plurality of series of measured intensity values using at least some of the intensity value thresholds.