公开(公告)号：US20200010888A1

公开(公告)日：2020-01-09

申请号：US16355607

申请日：2019-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ming JIANG ,  Chengyong YANG ,  Eugene WANG

IPC: C12Q1/6874

Abstract: A method for improving color calls or base calls utilizes current and prior cycle multi-channel intensity data from a sequencing run to model residual cycle buildup. The model is applied to correct the multi-cycle channel intensity for the current cycle. The corrected multi-cycle channel intensity is used for color calls or base calls for the current cycle.

公开(公告)号：US20130218476A1

公开(公告)日：2013-08-22

申请号：US13850287

申请日：2013-03-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Francis CHENG ,  Chengyong YANG ,  Casey McFARLAND ,  Ying WANG

IPC: G06F19/10 ,  G01N25/04

CPC classification number: G16B99/00 ,  C12Q1/68 ,  C12Q1/6827 ,  G01N21/64 ,  G01N25/04 ,  G01N25/48 ,  G16B40/00 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2563/173

Abstract: The present application provides for various embodiments of methods for the analysis of high resolution melt (HRM) curve data; where statistical assay variations in melt curve data may result from system noise in an analysis system. Such system noise may arise from various sources, such as the thermal non-uniformity of a thermocycler block in a thermal cycler apparatus, a detection system, etc. Additionally, various methods for the analysis of HRM curve data may provide an identification of a sample without the need for a user inputted information.

Abstract translation: 本申请提供了用于分析高分辨率熔体（HRM）曲线数据的方法的各种实施方案; 其中熔体曲线数据的统计测定变化可能由分析系统中的系统噪声引起。 这种系统噪声可能来自各种来源，例如热循环仪装置中的热循环器块的热不均匀性，检测系统等。另外，用于分析HRM曲线数据的各种方法可以提供样品的鉴定 而不需要用户输入的信息。

公开(公告)号：US20220367005A1

公开(公告)日：2022-11-17

申请号：US17805147

申请日：2022-06-02

Applicant: Life Technologies Corporation

Inventor： Ming JIANG ,  Chengyong YANG ,  Jianxin ZHANG

IPC: G16B30/10 ,  G16B30/00

Abstract: A method includes exposing template polynucleotide strands, sequencing primers, and polymerase to flows of nucleotide species; obtaining a series of measured intensity values and randomly selecting a training subset therefrom; generating series of base calls using a base caller and aligning the series of base calls to a reference genome or sequence using an aligner; determining intensity value thresholds and parameters of a linear transformation corresponding to different homopolymer lengths and nucleotide species; generating series of base calls corresponding to the series of measured intensity values using at least some of the parameters of a linear transformation; and recalibrating the series of base calls corresponding to the plurality of series of measured intensity values using at least some of the intensity value thresholds.

公开(公告)号：US20180104790A1

公开(公告)日：2018-04-19

申请号：US15652425

申请日：2017-07-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yerramalli Subramaniam ,  Puneet SURI ,  Sylvia CHANG ,  Chengyong YANG

IPC: B24B41/06 ,  B24B5/04

CPC classification number: B24B41/062 ,  B24B5/04 ,  B24B41/065 ,  G05B11/01 ,  G16B30/00

Abstract: Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. of various sequencing runs. In some embodiments, the system can also be in communication with a user interface, for example, a GUI, capable of representing and communicating various types of information to a user regarding the quality of the individual or multiple runs, the functioning of the instrument, an error event, etc. Additionally, the system can capable of receiving actionable information from a user via the GUI thereby allowing the user to terminate or repeat various sequencing steps in a particular run, terminate a entire run, terminate all runs, allow a run to proceed, etc.