公开(公告)号：US20130273544A1

公开(公告)日：2013-10-17

申请号：US13765677

申请日：2013-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu Li ,  Emily Zeringer ,  Richard Conrad

IPC: G01N1/34

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：US09347087B2

公开(公告)日：2016-05-24

申请号：US14523764

申请日：2014-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander Vlassov ,  Mu Li ,  Emily Zeringer ,  Richard Conrad

IPC: C07K1/30 ,  C12Q1/68 ,  G01N1/34 ,  G01N33/50

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。

公开(公告)号：US09671321B2

公开(公告)日：2017-06-06

申请号：US15142143

申请日：2016-04-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander Vlassov ,  Mu Li ,  Emily Zeringer ,  Richard Conrad

IPC: C07K1/30 ,  C12Q1/68 ,  G01N1/34 ,  G01N33/50

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：US08901284B2

公开(公告)日：2014-12-02

申请号：US14191109

申请日：2014-02-26

Applicant: Life Technologies Corporation

Inventor： Alexander Vlassov ,  Mu Li ,  Emily Zeringer ,  Richard Conrad

IPC: C07K1/30 ,  C12Q1/68 ,  G01N1/34 ,  G01N33/50

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。