公开(公告)号：US20150260680A1

公开(公告)日：2015-09-17

申请号：US14724354

申请日：2015-05-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John DAVIDSON ,  Wolfgang HINZ ,  Jonathan ROTHBERG ,  Richard WHITAKER

IPC: G01N27/414

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

公开(公告)号：US20170292156A1

公开(公告)日：2017-10-12

申请号：US15625800

申请日：2017-06-16

Applicant: Life Technologies Corporation

Inventor： John DAVIDSON ,  Wolfgang HINZ ,  Jonathan ROTHBERG ,  Richard WHITAKER

IPC: C12Q1/68 ,  C12N9/12 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.