公开(公告)号：US20230313272A1

公开(公告)日：2023-10-05

申请号：US18179184

申请日：2023-03-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chrisgen VONNEGUT ,  Scott CLARKE

IPC: C12Q1/6809

CPC classification number: C12Q1/6809 ,  C12Q2563/107 ,  C12Q2565/102

Abstract: Disclosed is a method for determining the quality, expressed in terms of a quality value, of an RNA sample based on a multiplex fluorescent ratiometric method, wherein the ratio of small RNA to large and/or structured RNA in a sample is determined, thereby obtaining the quality value. Also disclosed is a method for determining the quality value of an RNA sample wherein the relationship of the intensity of the fluorescent signal corresponding to small RNA is compared to the intensity of the fluorescent signal corresponding to large and/or structured RNA in a sample. The relationship of the intensity of the two fluorescent signals is used to determine the amount of intact RNA present in the sample.

公开(公告)号：US20190154696A1

公开(公告)日：2019-05-23

申请号：US15753454

申请日：2016-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott CLARKE ,  Kyle GEE ,  Carmen FINNESSY

IPC: G01N33/58 ,  C12Q1/6813 ,  G01N33/52

Abstract: The present disclosure provides methods, compositions and kits for measuring cellular nascent nucleic acid synthesis by colorimetric labeling of nucleic acid. The nucleic acid synthesis can be measured as cell proliferation, DNA or RNA synthesis, gene expression or apoptosis. Additionally, these methods may be used to screen compounds for their effect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

公开(公告)号：US20200224249A1

公开(公告)日：2020-07-16

申请号：US16649088

申请日：2018-10-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chris VONNEGUT ,  Scott CLARKE

IPC: C12Q1/6809

Abstract: Disclosed is a method for determining the quality, expressed in terms of a quality value, of an RNA sample based on a multiplex fluorescent ratiometric method, wherein the ratio of small RNA to large and/or structured RNA in a sample is determined, thereby obtaining the quality value. Also disclosed is a method for determining the quality value of an RNA sample wherein the relationship of the intensity of the fluorescent signal corresponding to small RNA is compared to the intensity of the fluorescent signal corresponding to large and/or structured RNA in a sample. The relationship of the intensity of the two fluorescent signals is used to determine the amount of intact RNA present in the sample.

公开(公告)号：US20160130637A1

公开(公告)日：2016-05-12

申请号：US14300728

申请日：2014-06-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott CLARKE ,  Jolene BRADFORD

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  C12Q1/6804 ,  C12Q1/6846 ,  C12Q2565/1025 ,  C12Q2525/101

Abstract: The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. Pulse labeling of the nucleic acid with a second nucleoside analog then allows measurement of any changes to nucleic acid synthesis. The nucleic acid synthesis can be measured as cell proliferation, DNA, or gene expression, RNA. This method does not require a potentially artifact-inducing intermediary wash step between pulse labels. Additionally, this method may be used to screen compounds for their affect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

Abstract translation: 本发明提供了一种通过核酸双重脉冲标记来测量细胞新生核酸合成的方法。 具有核苷类似物的核酸的第一次脉冲标记允许建立基线核酸合成速率。 用第二核苷类似物脉冲标记核酸，然后允许测量核酸合成的任何变化。 核酸合成可以作为细胞增殖，DNA或基因表达，RNA来测量。 该方法不需要在脉冲标签之间产生潜在的伪影中介洗涤步骤。 此外，该方法可用于通过用竞争性核苷类似物在治疗前或之前用受试化合物处理细胞或生物体来筛选化合物对细胞增殖的影响。