公开(公告)号：US11542535B2

公开(公告)日：2023-01-03

申请号：US14315140

申请日：2014-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhoutao Chen ,  Xiaoping Duan ,  Kyusung Park

IPC: C12Q1/68 ,  C12P19/40 ,  C12N15/10 ,  C12Q1/6806

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.