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    Bi-directional sequencing compositions and methods
    1.
    发明授权
    Bi-directional sequencing compositions and methods 有权

    公开(公告)号：US10508306B2

    公开(公告)日：2019-12-17

    申请号：US16123823

    申请日：2018-09-06

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

    IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

    Nucleic acid adaptors and uses thereof
    4.
    发明授权
    Nucleic acid adaptors and uses thereof 有权

    公开(公告)号：US10450608B2

    公开(公告)日：2019-10-22

    申请号：US15372938

    申请日：2016-12-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Bin Li ,  Zhoutao Chen ,  Tanya Biorac ,  Melvin Wei

    IPC: C12P19/34 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12N15/10 ,  C12N9/12 ,  C12N9/20 ,  C12Q1/6832 ,  C12N15/64

    Abstract: Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can comprise a double-stranded oligonucleotide adaptor (duplex) having an overhang cohesive portion that anneals with a blocking oligonucleotide which can be a separate single-stranded oligonucleotide. Also provided herein are compositions generated using the blocking oligonucleotide adapters, including circularized polynucleotides, that may be manipulated, for example, through nick translation, to yield a linear mate pair construct.

    Methods for manipulating biomolecules
    5.
    发明授权
    Methods for manipulating biomolecules 有权

    公开(公告)号：US11542535B2

    公开(公告)日：2023-01-03

    申请号：US14315140

    申请日：2014-06-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Zhoutao Chen ,  Xiaoping Duan ,  Kyusung Park

    IPC: C12Q1/68 ,  C12P19/40 ,  C12N15/10 ,  C12Q1/6806

    Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

    Bi-directional sequencing compositions and methods
    7.
    发明授权
    Bi-directional sequencing compositions and methods 有权
    双向测序组合物和方法

    公开(公告)号：US09587269B2

    公开(公告)日：2017-03-07

    申请号：US14832772

    申请日：2015-08-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

    IPC: C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

    Abstract translation: 在一些实施方案中，从与支持物连接的核酸模板获得序列信息的方法包括将第一引物与连接于载体的模板链杂交，对核酸模板的一部分进行测序，从而形成延伸的第一引物产物， 与一部分核酸模板互补。在一些实施方案中，该方法还包括将切口引入与延伸的第一引物产物杂交的模板链的一部分中，使用下述方法从模板中降解模板链的一部分： 其中一部分延伸的第一引物保留与模板链的未降解部分杂交，并通过合成测序延伸的第一引物的至少一些单链部分。

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