公开(公告)号：US20240425921A1

公开(公告)日：2024-12-26

申请号：US18787313

申请日：2024-07-29

Applicant: LIFE TECHNOLOGIES CORPORATION ,  ONE LAMBDA, INC.

Inventor： Rui PEI ,  Peter VANDER HORN ,  Hong MA ,  Ito WAKEFIELD

IPC: C12Q1/6876 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6844

Abstract: Methods, systems, and devices for analyzing a sample for the presence of a target protein are provided. A sample can be processed, the processing generating an amplification product of a template nucleic acid formed in response to a target protein being present in the sample. The amplification product can be contacted with a detection probe to generate a second product comprising a labeled nucleic acid. A lateral flow substrate can be contacted with the second product, the lateral flow substrate comprising a capture moiety configured to bind the labeled nucleic acid. The methods can be carried out in one or more chambers of a single device or multiple devices. Detection probe bound to amplicons of the template nucleic acid captured on the lateral flow substrate can be read visually or using a sensor.