公开(公告)号：US20230047838A1

公开(公告)日：2023-02-16

申请号：US17815465

申请日：2022-07-27

申请人： LONZA LTD

发明人： James BUDGE ,  Christopher Mark SMALES ,  Tanya Jeane KNIGHT ,  Robert YOUNG

IPC分类号： C12N9/02 ,  C12P21/02 ,  C07K14/47 ,  C07K16/00 ,  C12N5/071

摘要： The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

公开(公告)号：US20230174971A1

公开(公告)日：2023-06-08

申请号：US16966768

申请日：2019-02-01

申请人： LONZA LTD

发明人： Robert YOUNG ,  Christopher Mark SMALES ,  Colin Mark JAQUES ,  Andrew J. RACHER ,  Gurdeep SINGH ,  James BUDGE ,  Joanne ROBOOL

IPC分类号： C12N15/10 ,  C12N15/63

CPC分类号： C12N15/1075 ,  C12N15/63

摘要： Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.