公开(公告)号：US20110195505A1

公开(公告)日：2011-08-11

申请号：US12899760

申请日：2010-10-07

申请人： LORI JEAN EULER ,  DENNIS FLINT ,  BRIAN JAMES PAUL ,  TINA K. VAN DYK ,  RICK W. YE

发明人： LORI JEAN EULER ,  DENNIS FLINT ,  BRIAN JAMES PAUL ,  TINA K. VAN DYK ,  RICK W. YE

IPC分类号： C12N15/74 ,  C12N1/21 ,  C12N1/36

CPC分类号： C12P7/16 ,  C07K14/335 ,  C12N1/36 ,  Y02E50/10

摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the saturated fatty acids content in bacterial cell membrane was increased. Methods for increasing the concentration of saturated fatty acids in the membranes of bacteria that are not natural butanol produces are described whereby tolerance of the bacterial cell to butanol is increased. Saturated fatty acids concentration in the bacterial cell membrane increased upon exogenously feeding saturated fatty acids to cells. Bacterial strains useful for production of butanol are described herein having modified unsaturated fatty acid biosynthetic pathway.

摘要翻译： 当细菌细胞膜中的饱和脂肪酸含量增加时，发现不是天然丁醇生产者的细菌对丁醇具有增加的耐受性。 描述了增加非天然丁醇产生的细菌膜中饱和脂肪酸浓度的方法，其中细菌细胞对丁醇的耐受性增加。 饱和脂肪酸在细胞细胞膜中的浓度在将饱和脂肪酸外源供给细胞后增加。 本文描述了可用于生产丁醇的细菌菌株，其具有改性的不饱和脂肪酸生物合成途径。

公开(公告)号：US20100112655A1

公开(公告)日：2010-05-06

申请号：US12569136

申请日：2009-09-29

申请人： BRIAN JAMES PAUL

发明人： BRIAN JAMES PAUL

IPC分类号： C12P7/16 ,  C12N1/21

CPC分类号： C12N15/746 ,  C12N9/0006 ,  C12P7/16 ,  C12P7/18 ,  C12P7/26 ,  C12Y101/02004 ,  Y02E50/10

摘要： A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.

摘要翻译： 通过基因工程实现了植物乳杆菌中从丙酮酸到2,3-丁二醇的高通量代谢物。 在异源表达的丁二醇脱氢酶活性的存在下，大量消除乳酸脱氢酶活性导致2,3-丁二醇的产生，其至少是丙酮酸衍生产物总数的49％。

公开(公告)号：US20100081182A1

公开(公告)日：2010-04-01

申请号：US12569103

申请日：2009-09-29

申请人： BRIAN JAMES PAUL ,  Wonchul Suh

发明人： BRIAN JAMES PAUL ,  Wonchul Suh

IPC分类号： C12P7/16 ,  C12N1/21

CPC分类号： C12N9/88 ,  C12P7/16 ,  Y02E50/10

摘要： Lactic acid bacteria expressing dihydroxyacid dehydratase polypeptides with increased specific activity are disclosed. The lactic acid bacteria comprise recombinant genes encoding iron-sulfur cluster forming proteins.

摘要翻译： 公开了具有增加的比活性的表达二羟基酸脱水酶多肽的乳酸菌。 乳酸菌包含编码铁硫簇形成蛋白的重组基因。

公开(公告)号：US20100081183A1

公开(公告)日：2010-04-01

申请号：US12569168

申请日：2009-09-29

申请人： BRIAN JAMES PAUL ,  Wonchul Suh

发明人： BRIAN JAMES PAUL ,  Wonchul Suh

IPC分类号： C12P7/16 ,  C12N1/21

CPC分类号： C12N9/88 ,  C12P7/16 ,  Y02E50/10

摘要： Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 μmol min−1 mg−1. LAB cells with even higher activities of 0.2 to 0.6 μmol min−1 mg−1 of DHAD activity were obtained.These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.

摘要翻译： 改变乳酸菌（LAB）细胞使其具有增加至约0.1μmol/ min-1mg-1的二羟基酸脱水酶活性的比活性。 获得具有0.2至0.6μmol/ min-1mg-1的DHAD活性的更高活性的LAB细胞。 当额外的异丁醇生物合成途径酶被表达时，这些修饰的细胞可用于产生异丁醇。