公开(公告)号：US20110195505A1

公开(公告)日：2011-08-11

申请号：US12899760

申请日：2010-10-07

申请人： LORI JEAN EULER ,  DENNIS FLINT ,  BRIAN JAMES PAUL ,  TINA K. VAN DYK ,  RICK W. YE

发明人： LORI JEAN EULER ,  DENNIS FLINT ,  BRIAN JAMES PAUL ,  TINA K. VAN DYK ,  RICK W. YE

IPC分类号： C12N15/74 ,  C12N1/21 ,  C12N1/36

CPC分类号： C12P7/16 ,  C07K14/335 ,  C12N1/36 ,  Y02E50/10

摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the saturated fatty acids content in bacterial cell membrane was increased. Methods for increasing the concentration of saturated fatty acids in the membranes of bacteria that are not natural butanol produces are described whereby tolerance of the bacterial cell to butanol is increased. Saturated fatty acids concentration in the bacterial cell membrane increased upon exogenously feeding saturated fatty acids to cells. Bacterial strains useful for production of butanol are described herein having modified unsaturated fatty acid biosynthetic pathway.

摘要翻译： 当细菌细胞膜中的饱和脂肪酸含量增加时，发现不是天然丁醇生产者的细菌对丁醇具有增加的耐受性。 描述了增加非天然丁醇产生的细菌膜中饱和脂肪酸浓度的方法，其中细菌细胞对丁醇的耐受性增加。 饱和脂肪酸在细胞细胞膜中的浓度在将饱和脂肪酸外源供给细胞后增加。 本文描述了可用于生产丁醇的细菌菌株，其具有改性的不饱和脂肪酸生物合成途径。

公开(公告)号：US20090203097A1

公开(公告)日：2009-08-13

申请号：US12330534

申请日：2008-12-09

申请人： DENNIS FLINT ,  Robert A. Larossa ,  Vasantha Nagarajan ,  Tina K. Van Dyk ,  Rick W. Ye

发明人： DENNIS FLINT ,  Robert A. Larossa ,  Vasantha Nagarajan ,  Tina K. Van Dyk ,  Rick W. Ye

IPC分类号： C12P7/16 ,  C12N1/21 ,  C12N15/87

CPC分类号： C12P7/16 ,  C12N9/1007 ,  Y02E50/10

摘要： Screening of fatty acid fed bacteria which are not natural butanol producers identified increased membrane cyclopropane fatty acid as providing improved butanol tolerance. Increasing expression of cyclopropane fatty acid synthase in the presence of the enzyme substrate that is either endogenous to the cell or fed to the cell, increased butanol tolerance. Bacterial strains with increased cyclopropane fatty acid synthase and having a butanol biosynthetic pathway are useful for production of butanol.

摘要翻译： 筛选不是天然丁醇生产者的脂肪酸饲养细菌增加膜环丙烷脂肪酸提供改善的丁醇耐受性。 在酶底物存在下增加环丙烷脂肪酸合酶的表达，该酶底物是细胞内源的或进入细胞，提高了丁醇耐受性。 具有增加的环丙烷脂肪酸合成酶并具有丁醇生物合成途径的细菌菌株可用于生产丁醇。

公开(公告)号：US20120064561A1

公开(公告)日：2012-03-15

申请号：US13029558

申请日：2011-02-17

申请人： DENNIS FLINT ,  Brian James Paul ,  Rick W. Ye

发明人： DENNIS FLINT ,  Brian James Paul ,  Rick W. Ye

IPC分类号： C12N1/19 ,  C12P7/40 ,  C12P7/16 ,  C12Q1/25 ,  C12P7/04

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N9/88 ,  C12N15/81 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  C12Y402/01009 ,  G01N33/573 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

摘要翻译： 本发明涉及包含二羟基酸脱水酶多肽的重组宿主细胞，特别是酵母细胞。 本发明还涉及由于多肽的表达增加，细胞的Fe-S簇生物合成的调节或其组合而导致的具有增加的二羟基酸脱水酶多肽的比活性的重组宿主细胞。 本发明还包括使用宿主细胞的方法，以及用于鉴定增加宿主细胞中Fe-S簇生物合成途径中的通量的多肽的方法。

公开(公告)号：US20100136641A1

公开(公告)日：2010-06-03

申请号：US12625593

申请日：2009-11-25

申请人： DENNIS FLINT ,  TINA K. VAN DYK

发明人： DENNIS FLINT ,  TINA K. VAN DYK

IPC分类号： C12P7/16 ,  C12N1/20 ,  C12N15/74

CPC分类号： C12P7/16 ,  C12N1/38 ,  C12N9/90 ,  Y02E50/10

摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase.

摘要翻译： 发现当不饱和反式脂肪酸的膜含量增加时，不是天然丁醇生产者的细菌具有增加的对丁醇的耐受性。 用不饱和反式脂肪酸喂养细胞增加其在膜中的浓度，这也可以通过表达脂肪酸顺反异构酶来实现。

公开(公告)号：US20100081154A1

公开(公告)日：2010-04-01

申请号：US12569636

申请日：2009-09-29

申请人： DENNIS FLINT ,  Steven Cary Rothman ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

发明人： DENNIS FLINT ,  Steven Cary Rothman ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

IPC分类号： C12Q1/34 ,  C12N1/20 ,  C12N1/16 ,  C12P7/16 ,  C12P7/18

CPC分类号： G16B30/00 ,  C12N9/88 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12Y402/01009 ,  Y02E50/10

摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

摘要翻译： 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中表达为异源蛋白质，提供DHAD活性，用于将2,3-二羟基异戊酸酯转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化成α-酮戊二酸酯。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。

公开(公告)号：US20110313206A1

公开(公告)日：2011-12-22

申请号：US12966333

申请日：2010-12-13

申请人： GAIL K. DONALDSON ,  ANDREW C. ELIOT ,  DENNIS FLINT ,  LORI ANN MAGGIO-HALL ,  VASANTHA NAGARAJAN

发明人： GAIL K. DONALDSON ,  ANDREW C. ELIOT ,  DENNIS FLINT ,  LORI ANN MAGGIO-HALL ,  VASANTHA NAGARAJAN

IPC分类号： C12P7/16 ,  C07C31/12 ,  C12N1/15 ,  C12N1/21 ,  C12N1/19

CPC分类号： C12P7/16 ,  Y02E50/10

摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.