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    Methods of Modifying Eukaryotic Cells
    2.
    发明申请
    Methods of Modifying Eukaryotic Cells 审中-公开

    公开(公告)号：US20140033336A1

    公开(公告)日：2014-01-30

    申请号：US14046285

    申请日：2013-10-04

    申请人： ANDREW J. MURPHY ,  GEORGE D. YANCOPOULOS ,  MARGARET KAROW ,  LYNN MACDONALD ,  SEAN STEVENS

    发明人： ANDREW J. MURPHY ,  GEORGE D. YANCOPOULOS ,  MARGARET KAROW ,  LYNN MACDONALD ,  SEAN STEVENS

    IPC分类号： A01K67/027

    摘要： A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.

    Methods of Modifying Eukaryotic Cells
    5.
    发明申请
    Methods of Modifying Eukaryotic Cells 审中-公开

    公开(公告)号：US20140041068A1

    公开(公告)日：2014-02-06

    申请号：US14046291

    申请日：2013-10-04

    申请人： ANDREW J. MURPHY ,  GEORGE D. YANCOPOULOS ,  MARGARET KAROW ,  LYNN MACDONALD ,  SEAN STEVENS

    发明人： ANDREW J. MURPHY ,  GEORGE D. YANCOPOULOS ,  MARGARET KAROW ,  LYNN MACDONALD ,  SEAN STEVENS

    IPC分类号： A01K67/027

    摘要： A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.

    Common Light Chain Mouse
    7.
    发明申请
    Common Light Chain Mouse 审中-公开
    普通轻链鼠标

    公开(公告)号：US20110195454A1

    公开(公告)日：2011-08-11

    申请号：US13022759

    申请日：2011-02-08

    申请人： JOHN MCWHIRTER ,  LYNN MACDONALD ,  SEAN STEVENS ,  SAMUEL DAVIS ,  DAVID R. BUCKLER ,  ANDREW J. MURPHY

    发明人： JOHN MCWHIRTER ,  LYNN MACDONALD ,  SEAN STEVENS ,  SAMUEL DAVIS ,  DAVID R. BUCKLER ,  ANDREW J. MURPHY

    IPC分类号： C12P21/02 ,  A01K67/027

    CPC分类号： C07K16/22 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/515 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/30 ,  C12N15/8509 ,  C12N2800/204

    摘要： A genetically modified mouse is provided, wherein the mouse is incapable of rearranging and expressing an endogenous mouse immunoglobulin light chain variable sequence, wherein the mouse expresses only one or two human light chain variable domains encoded by human immunoglobulin sequences operably linked to the mouse kappa (κ) constant gene at the endogenous mouse κ locus, wherein the mouse expresses a reverse chimeric antibody having a light chain variable domain derived from one of only two human light chain variable region gene segments and a mouse κ constant domain, and a human heavy chain variable domain and a mouse heavy chain constant domain, from an endogenous mouse heavy chain locus. Bispecific epitope-binding proteins that are fully human are provided, comprising two different heavy chains that associate with an identical light chain that comprises a variable domain derived from one of two different human light chain variable region gene segments.

    摘要翻译： 提供遗传修饰的小鼠，其中小鼠不能重新排列并表达内源性小鼠免疫球蛋白轻链可变序列，其中小鼠仅表达由人免疫球蛋白序列编码的一个或两个可操作地连接到小鼠κ的人轻链可变结构域（ ＆kgr;）内源性小鼠的恒定基因 基因座，其中小鼠表达具有仅来自两个人轻链可变区基因片段之一的轻链可变结构域和小鼠kgr的逆嵌合抗体; 恒定结构域，以及来自内源小鼠重链基因座的人重链可变结构域和小鼠重链恒定结构域。 提供了完全人的双特异性表位结合蛋白，其包含两个不同的重链，所述重链与相同的轻链相关，其包含衍生自两个不同人轻链可变区基因片段之一的可变结构域。