公开(公告)号：US10532092B2

公开(公告)日：2020-01-14

申请号：US16027289

申请日：2018-07-04

申请人： Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences ,  Zhejiang University

发明人： Huichen Guo ,  Shiqi Sun ,  Ping Du ,  Zhidong Teng ,  Ruikang Tang ,  Ruibo Zhao ,  Jiaxi Ru ,  Yanquan Wei ,  Yun Zhang ,  Yuan Gao ,  Junwu Ma ,  Xiangtao Liu ,  Hong Yin

IPC分类号： A61K39/12 ,  A61K39/00

摘要： Foot-and-mouth disease virus like particles, the particles including a structural protein VP0, a structural protein VP1 including a mineralization peptide, a structural protein VP3, and a calcium phosphate coat. The structural protein VP1 including a mineralization peptide is encoded by a gene sequence represented by SEQ ID NO. 7, SEQ ID NO. 8 or SEQ ID NO. 9. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO. 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO. 3. The calcium phosphate coat covers the structural protein VP0, the structural protein VP1 including a mineralization peptide, and the structural protein VP3.

公开(公告)号：US10829741B2

公开(公告)日：2020-11-10

申请号：US15993637

申请日：2018-05-31

申请人： Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

发明人： Shiqi Sun ,  Huichen Guo ,  Yun Zhang ,  Yanyan Chang ,  Yanquan Wei ,  Jiaxi Ru ,  Xiaoying Zhi ,  Ping Du ,  Xiangtao Liu ,  Hong Yin ,  Jianxun Luo

IPC分类号： C12N7/04 ,  C12N7/00 ,  G01N33/569

摘要： A method for preparing serotype O foot-and-mouth disease virus-like particles, the method including: construction of small ubiquitin-like modifier fusion expression vector, construction of recombinant expression vectors, construction of recombinant co-expression vector, expression and purification of proteins, and in-vitro assembly of serotype O foot-and-mouth disease virus-like particles. The disclosure also provides a test strip for detecting serotype O foot-and-mouth disease including a bottom board, and a detection layer disposed on the top of the bottom board. A detection line and a control line are disposed on the detection layer. An absorbent layer is disposed at one end of the detection layer close to the control line, and an immuno-gold pad is disposed at the other side of the detection layer close to the detection line. A sample pad is disposed on the top of the immuno-gold pad.