公开(公告)号：US07175767B2

公开(公告)日：2007-02-13

申请号：US10549083

申请日：2004-04-01

申请人： Lars C. Andersson ,  Tesfai Gebru ,  Tania Ahmad ,  John Clachan ,  Andreas Axén

发明人： Lars C. Andersson ,  Tesfai Gebru ,  Tania Ahmad ,  John Clachan ,  Andreas Axén

IPC分类号： B01D15/08 ,  B01J20/26 ,  C07K1/14

CPC分类号： B01D15/3828 ,  B01J20/286 ,  B01J45/00 ,  C07C231/02

摘要： The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R—O—CO—(CH2)n—CHNH2—CO—OH wherein R is a selectively cleavable group; and n is an integer of 1–4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.

摘要翻译： 本发明涉及金属螯合分离介质的制备，其包括提供由下式定义的化合物：RO-CO-（CH 2）n - > 2 -CO-OH其中R是选择性可裂解基团; n为1-4的整数; 与乙醇反应; 衍生化 R的裂解; 将所得化合物固定至基础基质; 和羧酸的脱保护。 在一个实施方案中，第一步中提供的化合物是天冬氨酸或谷氨酸的乙酯，衍生化剂是溴乙酸乙酸乙酯。

公开(公告)号：US20060199950A1

公开(公告)日：2006-09-07

申请号：US10549083

申请日：2004-04-01

申请人： Lars Andersson ,  Tesfai Gebru ,  Tania Ahmad ,  John Clachan ,  Andreas Axén

发明人： Lars Andersson ,  Tesfai Gebru ,  Tania Ahmad ,  John Clachan ,  Andreas Axén

IPC分类号： C07K1/10 ,  B01J20/26

CPC分类号： B01D15/3828 ,  B01J20/286 ,  B01J45/00 ,  C07C231/02

摘要： The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R—O—CO—(CH2)n—CHNH2—CO—OH wherein R is a selectively cleavable group; and n is an integer of 1-4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.

摘要翻译： 本发明涉及金属螯合分离介质的制备，其包括提供由下式定义的化合物：RO-CO-（CH 2）n - > 2 -CO-OH其中R是选择性可裂解基团; n为1-4的整数; 与乙醇反应; 衍生化 R的裂解; 将所得化合物固定至基础基质; 和羧酸的脱保护。 在一个实施方案中，第一步中提供的化合物是天冬氨酸或谷氨酸的乙酯，衍生化剂是溴乙酸乙酸乙酯。

公开(公告)号：US07005071B2

公开(公告)日：2006-02-28

申请号：US10699058

申请日：2003-10-31

申请人： Lars C. Andersson ,  Tesfai Gebru ,  Andreas Axén ,  Jean-Luc Maloisel

发明人： Lars C. Andersson ,  Tesfai Gebru ,  Andreas Axén ,  Jean-Luc Maloisel

IPC分类号： B01D15/08

CPC分类号： B01J45/00 ,  B01D15/3828 ,  B01J20/286 ,  B01J20/3251 ,  B01J20/3265 ,  C07C323/58 ,  C07D333/36

摘要： The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatized scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

摘要翻译： 本发明涉及产生至少一种多齿金属螯合亲和配体的方法，该方法包括该步骤

公开(公告)号：US20100016564A1

公开(公告)日：2010-01-21

申请号：US12297241

申请日：2007-05-28

申请人： Lars C. Andersson ,  Makonnen Belew ,  Tesfai Gebru ,  Gunnar Glad ,  Helena Lindgren ,  Nils Norrman

发明人： Lars C. Andersson ,  Makonnen Belew ,  Tesfai Gebru ,  Gunnar Glad ,  Helena Lindgren ,  Nils Norrman

IPC分类号： C07K1/22 ,  C07H23/00

CPC分类号： B01J20/3265 ,  B01D15/3828 ,  B01J20/289 ,  B01J45/00

摘要： The present invention relates to a method of preparing an immobilised metal ion affinity chromatography (IMAC) adsorbent, which comprises to provide chromatography ligands comprised of alkylene diamine triacetic acid, or a derivative thereof, and coupling thereof to a carrier via nitrogen. In an advantageous embodiment, the alkylene diamine triacetic acid is ethylene diaminetriacetic acid (ED3A).

摘要翻译： 本发明涉及一种制备固定金属离子亲和层析（IMAC）吸附剂的方法，其包括提供由亚烷基二胺三乙酸或其衍生物组成的色谱配体，以及通过氮与载体的偶联。 在有利的实施方案中，亚烷基二胺三乙酸是乙二胺三乙酸（ED3A）。

公开(公告)号：US20060027501A1

公开(公告)日：2006-02-09

申请号：US11244603

申请日：2005-10-06

申请人： Lars Andersson ,  Ulf Tedebark ,  Tesfai Gebru ,  Andreas Axen ,  Jean-Luc Maloisel

发明人： Lars Andersson ,  Ulf Tedebark ,  Tesfai Gebru ,  Andreas Axen ,  Jean-Luc Maloisel

IPC分类号： B01D15/08

CPC分类号： B01J45/00 ,  B01D15/3828 ,  B01J20/286 ,  B01J20/3251 ,  B01J20/3265 ,  C07C323/58 ,  C07D333/36

摘要： The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of (a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; (b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatised scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

摘要翻译： 本发明涉及产生至少一种多齿金属螯合亲和配体的方法，该方法包括以下步骤：（a）提供至少一种包含羰基，相邻硫和亲核试剂的环状支架; （b）在保留支架的环状结构的同时，通过衍生所述支架的亲核试剂，在每个支架上提供至少一种多金属金属螯合亲和配体臂，任选地以金属螯合官能团的形式被保护; （c）通过加入一种将一种或多种金属螯合亲和配体臂添加到支架中的试剂，在衍生化支架的羰基和硫之间的键上开环; 如果需要，（d）使步骤（b）中提供的配体臂的功能脱保护。 在该方法的最优选实施方案中，步骤（c）和（d）同时作为一个步骤进行。