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    Method of generating metal chelating affinity ligands
    1.
    发明授权
    Method of generating metal chelating affinity ligands 有权
    产生金属螯合亲和配体的方法

    公开(公告)号：US07005071B2

    公开(公告)日：2006-02-28

    申请号：US10699058

    申请日：2003-10-31

    申请人： Lars C. Andersson ,  Tesfai Gebru ,  Andreas Axén ,  Jean-Luc Maloisel

    发明人： Lars C. Andersson ,  Tesfai Gebru ,  Andreas Axén ,  Jean-Luc Maloisel

    IPC分类号： B01D15/08

    CPC分类号： B01J45/00 ,  B01D15/3828 ,  B01J20/286 ,  B01J20/3251 ,  B01J20/3265 ,  C07C323/58 ,  C07D333/36

    摘要： The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatized scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

    摘要翻译： 本发明涉及产生至少一种多齿金属螯合亲和配体的方法，该方法包括该步骤

    Method of generating metal chelating affinity ligands
    2.
    发明申请
    Method of generating metal chelating affinity ligands 审中-公开
    产生金属螯合亲和配体的方法

    公开(公告)号：US20060027501A1

    公开(公告)日：2006-02-09

    申请号：US11244603

    申请日：2005-10-06

    申请人： Lars Andersson ,  Ulf Tedebark ,  Tesfai Gebru ,  Andreas Axen ,  Jean-Luc Maloisel

    发明人： Lars Andersson ,  Ulf Tedebark ,  Tesfai Gebru ,  Andreas Axen ,  Jean-Luc Maloisel

    IPC分类号： B01D15/08

    CPC分类号： B01J45/00 ,  B01D15/3828 ,  B01J20/286 ,  B01J20/3251 ,  B01J20/3265 ,  C07C323/58 ,  C07D333/36

    摘要： The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of (a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; (b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatised scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

    摘要翻译： 本发明涉及产生至少一种多齿金属螯合亲和配体的方法，该方法包括以下步骤：（a）提供至少一种包含羰基，相邻硫和亲核试剂的环状支架; （b）在保留支架的环状结构的同时，通过衍生所述支架的亲核试剂，在每个支架上提供至少一种多金属金属螯合亲和配体臂，任选地以金属螯合官能团的形式被保护; （c）通过加入一种将一种或多种金属螯合亲和配体臂添加到支架中的试剂，在衍生化支架的羰基和硫之间的键上开环; 如果需要，（d）使步骤（b）中提供的配体臂的功能脱保护。 在该方法的最优选实施方案中，步骤（c）和（d）同时作为一个步骤进行。

    NOVEL CHELATOR AND USE THEREOF
    4.
    发明申请
    NOVEL CHELATOR AND USE THEREOF 有权
    新任教士及其使用

    公开(公告)号：US20130072638A1

    公开(公告)日：2013-03-21

    申请号：US13701074

    申请日：2011-05-30

    申请人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

    发明人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

    IPC分类号： C08B37/00 ,  B01J20/281

    CPC分类号： C08B37/0039 ,  B01J20/281 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3265 ,  C07C237/10 ,  C07C323/60

    摘要： The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.The dimeric pentadentate chelator according to the invention has the following general formula: whereinSc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), andPD is a pentadentate chelator having the formula:

    摘要翻译： 本发明涉及具有非常强的金属离子结合的二聚五齿螯合物，用于生物分子的检测，固定和纯化。 二聚螯合剂提供了两个相邻的固定化金属离子与组氨酸标记的生物分子的结合的协同作用，与对应的单体螯合剂相比，其对结合强度具有有利的性质。 此外，二聚体增加了具有低金属离子亲和力的非标记生物分子的选择性（易于分离）。 根据本发明的二聚五齿螯合剂具有以下通式：其中Sc是含有至少两个能够偶合两种五齿螯合剂（PD）的官能团的支架或连接结构，PD是具有下式的五齿螯合剂：

    Method of preparing ligands for hydrophobic interaction chromatography
    9.
    发明申请
    Method of preparing ligands for hydrophobic interaction chromatography 失效
    制备疏水相互作用色谱配体的方法

    公开(公告)号：US20060175258A1

    公开(公告)日：2006-08-10

    申请号：US10547570

    申请日：2004-03-05

    申请人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    发明人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    IPC分类号： C07F5/04 ,  B01D15/08

    CPC分类号： B01J20/3285 ,  B01D15/325 ,  B01D15/327 ,  B01J20/286

    摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.

    摘要翻译： 本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。 本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。

    Method for the quantitative determination of one or more compounds
    10.
    发明申请
    Method for the quantitative determination of one or more compounds 审中-公开
    定量测定一种或多种化合物的方法

    公开(公告)号：US20050100878A1

    公开(公告)日：2005-05-12

    申请号：US10398439

    申请日：2001-10-02

    申请人： Bengt Bjellqvist ,  Jean-Luc Maloisel ,  Ronnie Palmgren ,  Jonas Astrom

    发明人： Bengt Bjellqvist ,  Jean-Luc Maloisel ,  Ronnie Palmgren ,  Jonas Astrom

    IPC分类号： G01N27/62 ,  G01N27/447 ,  G01N27/64 ,  G01N30/06 ,  G01N30/72 ,  G01N30/88 ,  G01N33/68 ,  C12Q1/00

    CPC分类号： G01N33/6803

    摘要： The present invention relates to a method for the quantitative determination of the amount of one or more biomolecules, such as proteins or polypeptides, in one or more samples by utilising sample unique tagging reagents. More specifically, the method comprises steps of providing at least two samples; reacting biomolecules present in each sample with a sample unique mass tagging reagent to provide sample unique mass tagged forms thereof; combining tagged forms present in each sample to provide a single sample; coseparating, from the resulting sample, a mix of mass tagged forms of each of said biomolecules into different fractions; subjecting, for each fraction, the mix to mass spectrometry to obtain a mass spectrum; and determining from signals in each mass spectrum, the amount of the biomolecule corresponding to the spectrum in at least one of said samples relative to the amount of the same biomolecule in at least one of the remaining samples. In an advantageous embodiment, the separation step is a gel electrophoresis step. In some cases, it may be advantageous to also include a step of digesting biomolecules, such as protein(s).

    摘要翻译： 本发明涉及通过利用样品独特标记试剂在一个或多个样品中定量测定一种或多种生物分子（例如蛋白质或多肽）的量的方法。 更具体地，该方法包括提供至少两个样本的步骤; 使每个样品中存在的生物分子与样品独特的质量标签试剂反应，以提供样品独特的质量标签形式; 组合每个样品中存在的标记形式以提供单个样品; 从所得样品中分离出每种所述生物分子的质量标签形式的混合物到不同的级分; 对每个级分进行混合物进行质谱分析以获得质谱; 并且从每个质谱图中的信号确定与至少一个剩余样品中相同生物分子的量相对应的至少一个所述样品中的光谱的生物分子的量。 在有利的实施方案中，分离步骤是凝胶电泳步骤。 在一些情况下，还可以包括消化生物分子如蛋白质的步骤是有利的。