公开(公告)号：US20110159551A1

公开(公告)日：2011-06-30

申请号：US12900794

申请日：2010-10-08

申请人： Lei (a.k.a Larry) Xi ,  Roland Nagel ,  Stephen Hendricks ,  Jennifer Berkman ,  Marian Peris ,  Yulei Wang

发明人： Lei (a.k.a Larry) Xi ,  Roland Nagel ,  Stephen Hendricks ,  Jennifer Berkman ,  Marian Peris ,  Yulei Wang

IPC分类号： C12P19/34 ,  C12N9/10

CPC分类号： C12N9/1276 ,  C12N15/1096 ,  C12Q2521/107 ,  C12Y207/07049

摘要： The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. The activity of the reverse transcriptase enzyme is recovered by an incubation of the reaction mixture at elevated temperature prior to, or as part of the reverse transcription reaction. The reverse transcriptase enzyme of the present invention provides for a signficant reduction in non-specific reverse transcription from template nucleic acid molecules because the formulation of the reaction mixture does not support the formation of reverse transcription products prior to activation of the reverse transcriptase.

摘要翻译： 本发明提供使用可逆失活的逆转录酶进行逆转录反应的组合物和方法。 可逆灭活的逆转录酶由化学修饰产生，其使逆转录酶失活。 通过在反转录反应之前或作为逆转录反应的一部分的升高温度下温育反应物来回收逆转录酶的活性。 本发明的逆转录酶提供了明显减少模板核酸分子的非特异性逆转录，因为反应混合物的配方不支持在逆转录酶激活之前形成逆转录产物。