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公开(公告)号：US4013511A
公开(公告)日：1977-03-22
申请号：US469043
申请日：1974-05-10
CPC分类号： C08F8/00
摘要： Enzymes are convalently found to anionic or cationic hydrophilic polymeric carriers formed by essentially complete reaction of pendent anhydride groups of ethylene-maleic anhydride copolymer (EMA) with a diamine selected from the group consisting of hydrazine, P, P'-diaminodiphenylmethane and 1,6-diaminohexane to produce resins having pendent amine groups. The EMA-hydrazide and EMA- P,P'-diaminodiphenylmethane resins can be rendered cationic by reaction with N,N-dimethyl-1,3-propanediamine.
摘要翻译： 通过乙烯 - 马来酸酐共聚物（EMA）的悬挂酸酐基团与选自肼，P，P'-二氨基二苯基甲烷和1,6二胺的二胺的基本上完全反应形成的阴离子或阳离子亲水性聚合物载体， - 二氨基己烷，生成具有侧基胺基的树脂。 EMA-酰肼和EMA-P，P'-二氨基二苯基甲烷树脂可以通过与N，N-二甲基-1,3-丙二胺反应而变成阳离子。
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公开(公告)号：US5143839A
公开(公告)日：1992-09-01
申请号：US563347
申请日：1990-08-07
摘要： The invention relates to highly purified aminopeptidases derived from Streptomycetes which are heat stable. There is further provided assay for the detection and quantitative determination of Neutral Endopeptidase 24.11 and Neutral Proteinase 24.4. A specific aminopeptidase is one derived from Streptomyces griseus, which is a calcium activated metalloprotein.
摘要翻译： 本发明涉及热稳定的源自链霉菌的高度纯化的氨基肽酶。 还进一步提供了中性内肽酶24.11和中性蛋白酶24.4的检测和定量测定。 特定的氨基肽酶是从灰质链霉菌（Screptomyces griseus）得到的，其是钙激活的金属蛋白酶。
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3.发明授权Method of removing N-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby 失效从由此产生的真核生物多肽类似物和多肽中除去N-末端氨基酸残基的方法
公开(公告)号：US5763215A
公开(公告)日：1998-06-09
申请号：US400544
申请日：1995-03-08
CPC分类号： C07K14/61
摘要： A method of sequentially removing one or more N-terminal amino acid residues from an analog of a eucaryotic polypeptide synthesized in a foreign host, comprises contacting the eucaryotic polypeptide analog with aminopeptidase under suitable conditions permitting sequential removal of N-terminal amino acid residues, where the polypeptide analog contains an amino acid residue or sequence of residues which blocks the action of an aminopeptidase located at a position other than the N-terminal end of the polypeptide analog. A specific embodiment of the invention concerns removing both an N-terminal methionine residue and its adjacent Leucine residue from a growth hormone analog produced in bacteria by contacting the growth hormone analog with Aeromonas aminopeptidase under suitable conditions permitting removal of the N-terminal methionine and Leucine residues. The invention also concerns eucaryotic polypeptide analogs produced in accordance with the methods of the invention.
摘要翻译： 从外来宿主合成的真核多肽的类似物中顺序地除去一个或多个N-末端氨基酸残基的方法包括在合适的条件下使真核多肽类似物与氨基肽酶接触，允许顺序除去N-末端氨基酸残基，其中 多肽类似物包含阻断位于多肽类似物的N-末端以外的位置的氨基肽酶的作用的氨基酸残基或残基序列。 本发明的具体实施方案涉及通过在合适的条件下使生长激素类似物与气单胞菌氨基肽酶接触，从而从细菌中产生的生长激素类似物中除去N-末端甲硫氨酸残基及其相邻的亮氨酸残基，从而允许除去N-末端甲硫氨酸和亮氨酸 残留物。 本发明还涉及根据本发明方法生产的真核生物多肽类似物。
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