Truncated form of fibrobacter succinogenes 1,3-1, 4-β-d-glucanase with improved enzymatic activity and thermo-tolerance
    6.
    发明授权
    Truncated form of fibrobacter succinogenes 1,3-1, 4-β-d-glucanase with improved enzymatic activity and thermo-tolerance 失效
    脆性纤维杆菌1,3-1,4-β-D-葡聚糖酶的截短形式,具有改善的酶活性和耐热性

    公开(公告)号:US07037696B1

    公开(公告)日:2006-05-02

    申请号:US09654652

    申请日:2000-09-05

    IPC分类号: C12N9/02

    摘要: A truncated glucanase with an improved thermal stability and a higher specific enzymatic activity than the wild-type enzyme. The truncated glucanase is obtained by removing a number of amino acid residues from the C-terminal of the wild-type 1,3-1,4-β-D-glucanase of Fibrobacter succinogenes. The removal of the C-terminal amino acid residues can be conducted at the genetic level by modifying the gene encoding for the wild type enzyme using, for example, a PCR-based method. Or, it can also be conducted at the protein level by first producing the wild-type enzyme protein and then subjecting the wild-type protein to certain protease action to remove a portion of its C-terminal.

    摘要翻译: 具有比野生型酶更高的热稳定性和更高的特异性酶活性的截短的葡聚糖酶。 截短的葡聚糖酶是通过从富含纤维杆菌的野生型1,3-1,4-β-D-葡聚糖酶的C末端除去多个氨基酸残基获得的。 通过使用例如基于PCR的方法修饰编码野生型酶的基因,可以在遗传水平上进行C-末端氨基酸残基的去除。 或者也可以在蛋白质水平上进行,首先制造野生型酶蛋白质,然后使野生型蛋白质进行某些蛋白酶作用去除其C末端的一部分。

    Dioscorea extracts
    9.
    发明申请
    Dioscorea extracts 有权
    薯a提取物

    公开(公告)号:US20060105070A1

    公开(公告)日:2006-05-18

    申请号:US11331401

    申请日:2006-01-12

    IPC分类号: A61K36/8945

    摘要: An extract from a tuber of a Dioscorea plant. Also disclosed is a composition containing an extract from a tuber of a Dioscorea plant and a cytokine, as well as methods of enhancing proliferation of bone marrow or spleen cells and stimulating regeneration of a specific lineage of hematopoietic cells.

    摘要翻译: 薯a植物的块茎提取物。 还公开了含有薯a植物和细胞因子的块茎的提取物的组合物,以及增强骨髓或脾细胞增殖并刺激造血细胞特异性谱系再生的方法。