公开(公告)号：US20140291576A1

公开(公告)日：2014-10-02

申请号：US14183999

申请日：2014-02-19

Applicant: Life Technologies AS

Inventor： Arne DEGGERDAL ,  Frank Larsen

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.

Abstract translation: 本发明提供了从样品中分离核酸的方法，所述方法包括使所述样品与洗涤剂和固体支持物接触，由此使所述样品中的可溶性核酸与载体结合，并将所述载体与结合的核酸从 例子。 当本发明的方法用于分离DNA时，可以方便地与进一步的步骤相结合以从同一样品中分离RNA。