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1.发明授权Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis 失效不对称呫吨荧光染料的倾向异构体和DNA测序和片段分析方法公开(公告)号：US06448407B1
公开(公告)日：2002-09-10
申请号：US09704966
申请日：2000-11-01
IPC分类号： C07D31182
摘要： Substantially pure atropisomers of xanthene compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
摘要翻译： 公开了呫吨化合物的基本纯的阻转异构体。 各种分子生物学应用利用无异构呫吨荧光染料作为底物的标记，例如核苷酸，核苷，多核苷酸，多肽和碳水化合物。 方法包括DNA测序，DNA片段分析，PCR，SNP分析，寡核苷酸连接，扩增，minisequencing和引物延伸。
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公开(公告)号：US07038063B2
公开(公告)日：2006-05-02
申请号：US10716165
申请日：2003-11-18
IPC分类号： C07D311/82
摘要： Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
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3.发明授权Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis 失效不对称呫吨荧光染料的倾向异构体和DNA测序和片段分析方法公开(公告)号：US06649769B2
公开(公告)日：2003-11-18
申请号：US10227058
申请日：2002-08-21
IPC分类号： C07D40304
摘要： Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
摘要翻译： 公开了异转移能量转移染料化合物。 各种分子生物学应用利用无异构呫吨荧光染料作为底物的标记，例如核苷酸，核苷，多核苷酸，多肽和碳水化合物。 方法包括DNA测序，DNA片段分析，PCR，SNP分析，寡核苷酸连接，扩增，minisequencing和引物延伸。
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公开(公告)号：US07687236B2
公开(公告)日：2010-03-30
申请号：US12240924
申请日：2008-09-29
摘要： Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.
摘要翻译： 提供的是核苷酸 - 染料缀合物和相关化合物，其中染料通过阴离子连接体直接或间接连接到核碱基。 连接体的阴离子特征由连接物中存在的一个或多个阴离子部分提供，例如磷酸酯，膦酸酯，磺酸酯和羧酸酯基团。 当染料作为供体/受体染料对提供时，阴离子连接体可以位于供体和受体之间，或位于核碱基与供体或受体之间或两者之间。 在一个实施方案中，本发明的缀合物为测序片段提供增强的电泳迁移率特性，例如使用标记的终止子进行双脱氧测序。
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公开(公告)号：US07323696B2
公开(公告)日：2008-01-29
申请号：US11031983
申请日：2005-01-06
CPC分类号： B82Y5/00 , C12Q2563/155 , G01N33/54346 , G01N33/587
摘要： Beads coded with phosphor particles and methods of making and using them are provided.
摘要翻译： 提供用磷光体颗粒编码的珠子，并制作和使用它们的方法。
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