公开(公告)号：US09657322B2

公开(公告)日：2017-05-23

申请号：US13319237

申请日：2010-05-10

申请人： Lubbert Dijkhuizen ,  Marc Jos Elise Cornelis van der Maarel ,  Johannis Paulus Kamerling ,  Reinder Johannes Leemhuis ,  Slavko Kralj ,  Justyna Malgorzata Dobruchowska

发明人： Lubbert Dijkhuizen ,  Marc Jos Elise Cornelis van der Maarel ,  Johannis Paulus Kamerling ,  Reinder Johannes Leemhuis ,  Slavko Kralj ,  Justyna Malgorzata Dobruchowska

IPC分类号： C12P19/04 ,  C12P19/00 ,  C12N9/10

CPC分类号： C12P19/00 ,  C12N9/1051 ,  C12P19/04 ,  C12Y204/01005

摘要： The invention relates to the field of poly-and oligosaccharides and their nutritional effects. In particular, it relates to the application of α-glucanotransferases in methods for preparing dietary fibers, including prebiotic oligosaccharides, and to novel oligosaccharides obtainable thereby. Provided is a method for producing a mixture of gluco-oligosaccharides having one or more consecutive (α1→6) glucosidic linkages and one or more consecutive (α1→4) glucosidic linkages, comprising contacting a poly- and/or oligosaccharide substrate comprising at least two (α1→4) linked D-glucose units with an α-glucanotransferase capable of cleaving (α1→4) glucosidic linkages and making new (α1→4) and (α1→6) glucosidic linkages. Also provided are (isolated) gluco-oligosaccharides obtainable thereby, and their application in nutritional and cosmetic compositions.

公开(公告)号：US20120165290A1

公开(公告)日：2012-06-28

申请号：US13319237

申请日：2010-05-10

申请人： Lubbert Dijkhuizen ,  Marc Jos Elise Cornelis van der Maarel ,  Johannis Paulus Kamerling ,  Reinder Johannes Leemhuis ,  Slavko Kralj ,  Justyna Malgorzata Dobruchowska

发明人： Lubbert Dijkhuizen ,  Marc Jos Elise Cornelis van der Maarel ,  Johannis Paulus Kamerling ,  Reinder Johannes Leemhuis ,  Slavko Kralj ,  Justyna Malgorzata Dobruchowska

IPC分类号： A61K8/60 ,  A23L2/52 ,  C12N9/10 ,  A23L1/308 ,  C07H3/06 ,  C12P19/00

CPC分类号： C12P19/00 ,  C12N9/1051 ,  C12P19/04 ,  C12Y204/01005

摘要： The invention relates to the field of poly- and oligosaccharides and their nutritional effects. In particular, it relates to the application of α-glucanotransferases in methods for preparing dietary fibers, including prebiotic oligosaccharides, and to novel oligosaccharides obtainable thereby. Provided is a method for producing a mixture of gluco-oligosaccharides having one or more consecutive (α1→6) glucosidic linkages and one or more consecutive (α1→4) glucosidic linkages, comprising contacting a poly- and/or oligosaccharide substrate comprising at least two (α1→4) linked D-glucose units with an ?-glucanotransferase capable of cleaving (α1→4) glucosidic linkages and making new (α1→4) and (α1→6) glucosidic linkages. Also provided are (isolated) gluco-oligosaccharides obtainable thereby, and their application in nutritional and cosmetic compositions.

摘要翻译： 本发明涉及多糖和寡糖的领域及其营养效果。 特别涉及α-葡聚糖转移酶在制备膳食纤维，包括益生元寡糖的方法中的应用，以及由此获得的新型寡糖。 本发明提供一种具有一个或多个连续（α1→6）糖苷键和一个或多个连续（α1→4）糖苷键的葡萄糖寡糖的混合物的方法，包括使包含至少包含（α1→6）糖苷键的多糖和/或寡糖底物 两个（α1→4）连接的D-葡萄糖单元与能够切割（α1→4）糖苷键的β-葡聚糖转移酶并制备新的（α1→4）和（α1→6）糖苷键。 还提供了可由此获得的（分离的）葡糖寡糖，以及它们在营养和美容组合物中的应用。