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公开(公告)号：US09657322B2
公开(公告)日：2017-05-23
申请号：US13319237
申请日：2010-05-10
申请人： Lubbert Dijkhuizen , Marc Jos Elise Cornelis van der Maarel , Johannis Paulus Kamerling , Reinder Johannes Leemhuis , Slavko Kralj , Justyna Malgorzata Dobruchowska
发明人： Lubbert Dijkhuizen , Marc Jos Elise Cornelis van der Maarel , Johannis Paulus Kamerling , Reinder Johannes Leemhuis , Slavko Kralj , Justyna Malgorzata Dobruchowska
CPC分类号： C12P19/00 , C12N9/1051 , C12P19/04 , C12Y204/01005
摘要： The invention relates to the field of poly-and oligosaccharides and their nutritional effects. In particular, it relates to the application of α-glucanotransferases in methods for preparing dietary fibers, including prebiotic oligosaccharides, and to novel oligosaccharides obtainable thereby. Provided is a method for producing a mixture of gluco-oligosaccharides having one or more consecutive (α1→6) glucosidic linkages and one or more consecutive (α1→4) glucosidic linkages, comprising contacting a poly- and/or oligosaccharide substrate comprising at least two (α1→4) linked D-glucose units with an α-glucanotransferase capable of cleaving (α1→4) glucosidic linkages and making new (α1→4) and (α1→6) glucosidic linkages. Also provided are (isolated) gluco-oligosaccharides obtainable thereby, and their application in nutritional and cosmetic compositions.
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2.发明申请GLUCOOLIGOSACCHARIDES COMPRISING (ALPHA 1->4) AND (ALPHA 1->6) GLYCOSIDIC BONDS, USE THEREOF, AND METHODS FOR PROVIDING THEM 有权包含（ALPHA 1-> 4）和（ALPHA 1-> 6）GLYCOSIDIC BONDS的GLUCOOLIGOSACCHARIDES及其用途及其提供方法公开(公告)号：US20120165290A1
公开(公告)日：2012-06-28
申请号：US13319237
申请日：2010-05-10
申请人： Lubbert Dijkhuizen , Marc Jos Elise Cornelis van der Maarel , Johannis Paulus Kamerling , Reinder Johannes Leemhuis , Slavko Kralj , Justyna Malgorzata Dobruchowska
发明人： Lubbert Dijkhuizen , Marc Jos Elise Cornelis van der Maarel , Johannis Paulus Kamerling , Reinder Johannes Leemhuis , Slavko Kralj , Justyna Malgorzata Dobruchowska
CPC分类号： C12P19/00 , C12N9/1051 , C12P19/04 , C12Y204/01005
摘要： The invention relates to the field of poly- and oligosaccharides and their nutritional effects. In particular, it relates to the application of α-glucanotransferases in methods for preparing dietary fibers, including prebiotic oligosaccharides, and to novel oligosaccharides obtainable thereby. Provided is a method for producing a mixture of gluco-oligosaccharides having one or more consecutive (α1→6) glucosidic linkages and one or more consecutive (α1→4) glucosidic linkages, comprising contacting a poly- and/or oligosaccharide substrate comprising at least two (α1→4) linked D-glucose units with an ?-glucanotransferase capable of cleaving (α1→4) glucosidic linkages and making new (α1→4) and (α1→6) glucosidic linkages. Also provided are (isolated) gluco-oligosaccharides obtainable thereby, and their application in nutritional and cosmetic compositions.
摘要翻译： 本发明涉及多糖和寡糖的领域及其营养效果。 特别涉及α-葡聚糖转移酶在制备膳食纤维，包括益生元寡糖的方法中的应用，以及由此获得的新型寡糖。 本发明提供一种具有一个或多个连续（α1→6）糖苷键和一个或多个连续（α1→4）糖苷键的葡萄糖寡糖的混合物的方法，包括使包含至少包含（α1→6）糖苷键的多糖和/或寡糖底物 两个（α1→4）连接的D-葡萄糖单元与能够切割（α1→4）糖苷键的β-葡聚糖转移酶并制备新的（α1→4）和（α1→6）糖苷键。 还提供了可由此获得的（分离的）葡糖寡糖，以及它们在营养和美容组合物中的应用。
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公开(公告)号：US09447400B2
公开(公告)日：2016-09-20
申请号：US13510902
申请日：2010-11-19
申请人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Wilhelmus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev , Wilhelmus Antonious Hendricus Van Der Kley , Johannes Franciscus Thomas Van Lieshout , Sander Van Stigt Thans , Gudrun Vogtentanz , Mats Sandgren
发明人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Wilhelmus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev , Wilhelmus Antonious Hendricus Van Der Kley , Johannes Franciscus Thomas Van Lieshout , Sander Van Stigt Thans , Gudrun Vogtentanz , Mats Sandgren
CPC分类号： C12N9/2445 , C12N15/52 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
摘要翻译： 本公开通常涉及酶，特别是β-葡糖苷酶变体。 还描述了编码β-葡糖苷酶变体的核酸，包含β-葡糖苷酶变体的组合物，使用β-葡糖苷酶变体的方法和鉴定另外有用的β-葡糖苷酶变体的方法。
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4.发明授权Cellulase variants with improved expression, activity and stability, and use thereof 有权具有改善的表达，活性和稳定性的纤维素酶变体及其用途公开(公告)号：US08679816B2
公开(公告)日：2014-03-25
申请号：US13321928
申请日：2010-06-03
申请人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W. J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
发明人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W. J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有改善的表达，活性和/或稳定性的纤维素酶变体。 还描述了编码纤维素酶变体的核酸，包含纤维素酶变体的组合物及其使用方法。
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公开(公告)号：US20130143301A1
公开(公告)日：2013-06-06
申请号：US13510902
申请日：2010-11-19
申请人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev
发明人： Richard R. Bott , Thijs Kaper , Bradley Kelemen , Frits Goedegebuur , Ronaldus Hommes , Slavko Kralj , Paulien Kruithof , Igor Nikolaev
IPC分类号： C12N9/24
CPC分类号： C12N9/2445 , C12N15/52 , C12P19/02 , C12P19/14 , C12Y302/01021
摘要： The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
摘要翻译： 本公开通常涉及酶，特别是β-葡糖苷酶变体。 还描述了编码β-葡糖苷酶变体的核酸，包含β-葡糖苷酶变体的组合物，使用β-葡糖苷酶变体的方法和鉴定另外有用的β-葡糖苷酶变体的方法。
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6.发明申请CELLULASE VARIANTS WITH IMPROVED EXPRESSION, ACTIVITY AND STABILITY, AND USE THEREOF 有权具有改进的表达，活性和稳定性的纤维素酶变体及其用途公开(公告)号：US20120135500A1
公开(公告)日：2012-05-31
申请号：US13321928
申请日：2010-06-03
申请人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W.J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
发明人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W.J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有改善的表达，活性和/或稳定性的纤维素酶变体。 还描述了编码纤维素酶变体的核酸，包含纤维素酶变体的组合物及其使用方法。
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