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1.发明申请SUBSTITUTED 6-ANILINOPURINE DERIVATIVES AS INHIBITORS OF CYTOKININ OXIDASE/DEHYDROGENASE AND PREPARATIONS CONTAINING THESE DERIVATIVES 有权作为细胞因子氧化酶/脱氢酶抑制剂和含有这些衍生物的制剂的6-硝基嘌呤衍生物公开(公告)号：US20100190806A1
公开(公告)日：2010-07-29
申请号：US12666189
申请日：2008-07-02
申请人： Lukas Spichal , Marketa Gemrotova , Marek Zatloukal , Jitka Frebortova , Petr Galuszka , Tomas Werner , Thomas Schmulling , Karel Dolezal , Miroslav Strnad
发明人： Lukas Spichal , Marketa Gemrotova , Marek Zatloukal , Jitka Frebortova , Petr Galuszka , Tomas Werner , Thomas Schmulling , Karel Dolezal , Miroslav Strnad
IPC分类号： A61K31/52 , C07D473/00
CPC分类号： C07D473/16 , A01N43/90 , C07D473/34
摘要： The invention relates to substituted 6-anilinopurine derivatives of the general formula I, wherein R denotes one to five substituents independently selected from the group consisting of hydrogen, halogen, hydroxyl, amino, alkyloxy and alkyl group, and R2 denotes amino, halogen, nitro, thio, alkylthio or alkyl group for use as inhibitors of cytokinin oxidase/dehydrogenase. The invention also relates to the compositions containing these derivatives.
摘要翻译： 本发明涉及通式I的取代的6-苯胺基嘌呤衍生物，其中R表示一至五个独立地选自氢，卤素，羟基，氨基，烷氧基和烷基的取代基，R2表示氨基，卤素，硝基 ，硫代，烷硫基或烷基，用作细胞分裂素氧化酶/脱氢酶的抑制剂。 本发明还涉及含有这些衍生物的组合物。
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2.发明授权Substituted 6-anilinopurine derivatives as inhibitors of cytokinin oxidase/dehydrogenase and preparations containing these derivatives 有权取代的6-苯胺嘌呤衍生物作为细胞分裂素氧化酶/脱氢酶的抑制剂和含有这些衍生物的制剂公开(公告)号：US08222260B2
公开(公告)日：2012-07-17
申请号：US12666189
申请日：2008-07-02
申请人： Lukas Spichal , Marketa Gemrotova , Marek Zatloukal , Jitka Frebortova , Petr Galuszka , Tomas Werner , Thomas Schmulling , Karel Dolezal , Miroslav Strnad
发明人： Lukas Spichal , Marketa Gemrotova , Marek Zatloukal , Jitka Frebortova , Petr Galuszka , Tomas Werner , Thomas Schmulling , Karel Dolezal , Miroslav Strnad
IPC分类号： A61K31/52 , C07D473/00
CPC分类号： C07D473/16 , A01N43/90 , C07D473/34
摘要： The invention relates to substituted 6-anilinopurine derivatives of the general formula I, wherein R denotes one to five substituents independently selected from the group consisting of hydrogen, halogen, hydroxyl, amino, alkyloxy and alkyl group, and R2 denotes amino, halogen, nitro, thio, alkylthio or alkyl group for use as inhibitors of cytokinin oxidase/dehydrogenase. The invention also relates to the compositions containing these derivatives.
摘要翻译： 本发明涉及通式I的取代的6-苯胺基嘌呤衍生物，其中R表示一至五个独立地选自氢，卤素，羟基，氨基，烷氧基和烷基的取代基，R2表示氨基，卤素，硝基 ，硫代，烷硫基或烷基，用作细胞分裂素氧化酶/脱氢酶的抑制剂。 本发明还涉及含有这些衍生物的组合物。
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3.发明申请Substituted 6-(alkylbenzylamino)purine derivatives for use as cytokinin receptor antagonists and preparations containing these derivatives 审中-公开用作细胞分裂素受体拮抗剂和含有这些衍生物的制剂的6-（烷基苄基氨基）嘌呤衍生物公开(公告)号：US20100240537A1
公开(公告)日：2010-09-23
申请号：US12680762
申请日：2008-10-03
申请人： Lukas Spichal , Igor Popa , Jiri Voller , Karel Dolezal , Miroslav Strnad , Tomas Werner , Thomas Schmulling
发明人： Lukas Spichal , Igor Popa , Jiri Voller , Karel Dolezal , Miroslav Strnad , Tomas Werner , Thomas Schmulling
IPC分类号： A01N43/90 , C07D473/34
CPC分类号： A01N43/90 , C07D473/34
摘要： The invention relates to 6-(alkylbenzylamino)purine derivatives of the general formula I for use as cytokinin receptor antagonists, wherein R1 is selected from the group comprising hydroxyl, amino, nitro, thio and alkyl group, and R2 denotes one to four alkyl groups. The invention also relates to preparations containing these derivatives.
摘要翻译： 本发明涉及用作细胞分裂素受体拮抗剂的通式I的6-（烷基苄基氨基）嘌呤衍生物，其中R1选自羟基，氨基，硝基，硫代和烷基，R2表示一至四个烷基 。 本发明还涉及含有这些衍生物的制剂。
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公开(公告)号：US20090222948A1
公开(公告)日：2009-09-03
申请号：US12291376
申请日：2008-11-07
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N9/0032 , C12N15/8261 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences. The use of these sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology, is also provided. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins as well as the use of such proteins and/or compounds as plant growth regulators or herbicides.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根形成和/或改变根向地力的方法，包括细胞分裂素氧化酶的表达或包括降低植物或植物中活性细胞分裂素水平的另一种蛋白质的表达 部分。 本发明还提供了增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 该方法包括细胞分裂素氧化酶的表达或另一种降低植物或植物部分活性细胞分裂素水平的蛋白质的表达。 还提供了用于提高种子产量的方法和组合物。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。 这些序列用于改善根相关特征，包括增加产量和/或增强早期活力和/或改善根/芽比，和/或改善抗倒伏和/或增加耐旱性和/或促进外植体的体外繁殖 和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物以及使用这些蛋白质和/或化合物作为植物生长调节剂或除草剂的方法。
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公开(公告)号：US20050150012A1
公开(公告)日：2005-07-07
申请号：US10498168
申请日：2002-12-10
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N9/0032 , C12N15/8295
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a plant cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology. The invention also relates to the use of said sequences in the above-mentioned methods. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins. The invention also relates to the use of said compounds as a plant growth regulator or herbicide.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根的形成和/或改变根地貌的方法，包括植物细胞分裂素氧化酶的表达或包括降低植物中活性细胞分裂素水平的另一种蛋白质的表达或 植物部位。 本发明还提供了增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 所述方法包括植物细胞分裂素氧化酶的表达或降低植物或植物部分中活性细胞分裂素水平的另一种蛋白质的表达。 本发明还涉及新型植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含所述序列的载体，宿主细胞，转基因细胞和植物。 本发明还涉及所述序列用于改善根相关特征的用途，包括增加产量和/或增强早期活力和/或改善根/芽比率和/或改善抗倒伏和/或增加耐旱性和/或促进作用 外植体的体外繁殖和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及上述方法中所述序列的用途。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物的方法。 本发明还涉及所述化合物作为植物生长调节剂或除草剂的用途。
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公开(公告)号：US20080222755A1
公开(公告)日：2008-09-11
申请号：US11801018
申请日：2007-05-08
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N15/8261 , C12N9/0032 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention provides nucleotide sequences and corresponding amino acid sequences for plant cytokinin oxidase proteins. In addition, vectors, host cells, and transgenic plants comprising such sequences as well as methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism using such sequences are provided by the present invention. Also provided by the present invention are methods for altering various plant phenotypes including delaying onset to flowering, increasing leaf thickness, reducing vessel size, inducing parthenocarpy, increasing branching, increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight using cytokinin oxidase proteins and/or nucleic acid molecules encoding cytokinin oxidase.
摘要翻译： 本发明提供植物细胞分裂素氧化酶蛋白质的核苷酸序列和相应的氨基酸序列。 此外，本发明提供了包含这些序列的载体，宿主细胞和转基因植物以及用于刺激根生长和/或增强侧向或不定根形成和/或使用这种序列改变根向上性的方法。 本发明还提供了改变各种植物表型的方法，包括延迟开花开始，增加叶厚度，减少血管大小，诱导单性结实，增加分枝，增加种子大小和/或重量，胚胎大小和/或重量，以及子叶 使用细胞分裂素氧化酶蛋白和/或编码细胞分裂素氧化酶的核酸分子的大小和/或重量。
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公开(公告)号：US20050223429A1
公开(公告)日：2005-10-06
申请号：US11057473
申请日：2005-02-14
CPC分类号： C12N9/0032 , C12N15/8229 , C12N15/823 , C12N15/8261 , C12N15/827 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences. The use of these sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology, is also provided. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins as well as the use of such proteins and/or compounds as plant growth regulators or herbicides.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根形成和/或改变根向地力的方法，包括细胞分裂素氧化酶的表达或包括降低植物或植物中活性细胞分裂素水平的另一种蛋白质的表达 部分。 本发明还提供了增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 该方法包括细胞分裂素氧化酶的表达或另一种降低植物或植物部分活性细胞分裂素水平的蛋白质的表达。 还提供了用于提高种子产量的方法和组合物。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。 这些序列用于改善根相关特征，包括增加产量和/或增强早期活力和/或改善根/芽比，和/或改善抗倒伏和/或增加耐旱性和/或促进外植体的体外繁殖 和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物以及使用这些蛋白质和/或化合物作为植物生长调节剂或除草剂的方法。
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8.发明申请Method for Modifying Plant Morphology, Biochemistry and Physiology Comprising Expression of Cytokinin Oxydase in the Seeds 审中-公开修饰种子中细胞分裂素氧化酶表达的植物形态，生物化学和生理学的方法公开(公告)号：US20080295197A1
公开(公告)日：2008-11-27
申请号：US11630012
申请日：2005-06-20
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N15/8261 , C12N9/0032 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention relates to methods and compositions for increasing seed yield of a plant. The methods comprise expression of a cytokinin oxidase in the aleurone and/or embryo of a seed. The invention also relates to vectors comprising a nucleic acid encoding a cytokinin oxidase that is operably linked to a promoter capable of driving expression in the aleurone and/or embryo of a seed, and to host cells, transgenic cells and plants comprising such sequences. The use of these sequences for increasing yield is also provided.
摘要翻译： 本发明涉及用于提高植物种子产量的方法和组合物。 所述方法包括在种子的糊粉和/或胚胎中表达细胞分裂素氧化酶。 本发明还涉及包含编码细胞分裂素氧化酶的核酸的载体，其与可驱动种子的糊粉和/或胚胎中的表达的启动子有效连接，以及宿主细胞，转基因细胞和包含该序列的植物。 还提供了这些序列用于增加产量的用途。
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公开(公告)号：US08859854B2
公开(公告)日：2014-10-14
申请号：US13539876
申请日：2012-07-02
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N15/8261 , C12N9/0032 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention provides methods and compositions for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences.
摘要翻译： 本发明提供了用于增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法和组合物。 该方法包括细胞分裂素氧化酶的表达或另一种降低植物或植物部分活性细胞分裂素水平的蛋白质的表达。 还提供了用于提高种子产量的方法和组合物。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。
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公开(公告)号：US08766038B2
公开(公告)日：2014-07-01
申请号：US11803820
申请日：2007-05-16
申请人： Thomas Schmulling , Tomas Werner
发明人： Thomas Schmulling , Tomas Werner
CPC分类号： C12N15/8261 , C12N9/0032 , C12N15/8271 , C12N15/8287 , C12N15/8295 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology. The invention also relates to the use of said sequences in the above-mentioned methods. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins. The invention also relates to the use of said compounds as a plant growth regulator or herbicide.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根的形成和/或改变根地貌的方法，包括植物细胞分裂素氧化酶的表达或包括降低植物中活性细胞分裂素水平的另一种蛋白质的表达或 植物部位。 本发明还涉及新型植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含所述序列的载体，宿主细胞，转基因细胞和植物。 本发明还涉及所述序列用于改善根相关特征的用途，包括增加产量和/或增强早期活力和/或改善根/芽比率和/或改善抗倒伏和/或增加耐旱性和/或促进作用 外植体的体外繁殖和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及上述方法中所述序列的用途。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物的方法。 本发明还涉及所述化合物作为植物生长调节剂或除草剂的用途。
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