专利检索 ap:("MACROGEN, INC." OR "PSOMAGEN, INC.") AND inv:"Hyung Il Lee" 第 1 页

1.

发明授权
DNA extraction method using microwave for next generation sequencing and use thereof 有权

公开(公告)号：US11781132B2

公开(公告)日：2023-10-10

申请号：US16830565

申请日：2020-03-26

申请人： MACROGEN, INC. , PSOMAGEN, INC.

发明人： Joshua Sungwoo Yang , Jaekyung Chon , Ik Jung Choi , Hyun Min Park , Jieun Park , Jeongsun Seo , Changhoon Kim , Jong Yeon Shin , Han Sol Seo , Jiwon Shin , In Hee Hwang , Seon Hye Sim , Chang Woo Cho , Kyuin Hwang , In Seon Kim , Hyung Il Lee , Jung Hyun Cho

IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6844

CPC分类号： C12N15/1082 , C12N15/1003 , C12Q1/6806 , C12Q1/6844

摘要： Disclosed are a method for DNA extraction in a sample for next generation sequencing (NGS) and a method of constructing a NGS library using the extracted DNA. The method for DNA extraction includes: preparing a mixture by mixing a biological sample with a buffer; applying microwaves to the mixture; and recovering DNA. The method of constructing a NGS library includes: extracting DNA according to the method for DNA extraction; amplifying a target DNA using primers; and purifying the amplified product and subjecting the purified product to library pooling.