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公开(公告)号：US10851395B2
公开(公告)日：2020-12-01
申请号：US15178890
申请日：2016-06-10
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/64
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.
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公开(公告)号：US20240060112A1
公开(公告)日：2024-02-22
申请号：US18490398
申请日：2023-10-19
IPC分类号： C12Q1/06 , C12N1/10 , C12N1/04 , C12P7/6409 , C12P7/6427
CPC分类号： C12Q1/06 , C12N1/10 , C12N1/04 , C12P7/6409 , C12P7/6427
摘要： Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.
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公开(公告)号：US20200332338A1
公开(公告)日：2020-10-22
申请号：US16864128
申请日：2020-04-30
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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公开(公告)号：US20180208954A1
公开(公告)日：2018-07-26
申请号：US15926680
申请日：2018-03-20
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , C12P7/6463
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.
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公开(公告)号：US11959120B2
公开(公告)日：2024-04-16
申请号：US17083045
申请日：2020-10-28
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/6427 , C12P7/6463
CPC分类号： C12P7/6427 , C12P7/6463
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.
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公开(公告)号：US11345943B2
公开(公告)日：2022-05-31
申请号：US16864128
申请日：2020-04-30
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
IPC分类号： C12P7/64 , C12Q1/06 , C12P7/6463 , C12P7/6472 , C12N1/12 , C12N1/14 , C12N1/04 , C12P7/6409 , C12P7/6427
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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公开(公告)号：US10676775B2
公开(公告)日：2020-06-09
申请号：US14882734
申请日：2015-10-14
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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公开(公告)号：US20160108452A1
公开(公告)日：2016-04-21
申请号：US14882734
申请日：2015-10-14
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
摘要翻译： 本文提供培养微生物的方法。 所述方法包括在介质中提供包含一种或多种具有第一碳氮比的微生物的容器; 培养微生物直到培养物达到阈值指标; 收集文化的一部分，同时保持容器中的大部分文化; 以及将包含第二碳氮比的新鲜培养基添加到容器中，其中大部分培养物包含微生物。
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公开(公告)号：US20220356506A1
公开(公告)日：2022-11-10
申请号：US17728833
申请日：2022-04-25
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
IPC分类号： C12Q1/06 , C12P7/6463 , C12P7/6472 , C12N1/12 , C12N1/14 , C12N1/04 , C12P7/6409 , C12P7/6427
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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公开(公告)号：US20210123083A1
公开(公告)日：2021-04-29
申请号：US17139817
申请日：2020-12-31
摘要： Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.
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