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公开(公告)号：US11466297B2
公开(公告)日：2022-10-11
申请号：US17395459
申请日：2021-08-06
IPC分类号： C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Provided herein are methods for producing one or more polyunsaturated fatty acids. The methods include the steps of providing a microorganism capable of producing polyunsaturated fatty acids, providing a medium comprising a high concentration of one or more carbon sources, low pH, or both, and culturing the microorganism in the medium under sufficient conditions to produce the one or more polyunsaturated fatty acids. Also provided are methods of culturing one or more microorganisms. The methods include culturing the microorganisms in a medium comprising a first amount of one or more carbon sources at a first concentration level, monitoring a carbon source concentration until the carbon source concentration is reduced below the first concentration level, and adding to the medium a second amount of one or more carbon sources to increase the carbon source concentration to a second concentration level.
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公开(公告)号：US20210388312A1
公开(公告)日：2021-12-16
申请号：US17286449
申请日：2019-10-21
发明人： Kimberly Hyson , Nathalia Florez , Denise Muise , Alexandra Merkx-Jacques , Dorothy Dennis , David Woodhall , Zachary Sun , Roberto E. Armenta
摘要： Provided herein are methods of making microorganisms modified for increased xylose consumption as compared to unmodified microorganisms. The methods include providing xylose-consuming microorganisms comprising two or more copies of a nucleic acid sequence encoding xylose isomerase and two or more copies of a nucleic acid sequence encoding a xylose kinase, culturing the microorganisms in medium containing xylose and harvesting a portion of the microorganisms. These steps are repeated multiple times. The microorganisms are then isolated. The isolated microorganisms have increased xylose consumption rates compared to control xylose-consuming microorganisms. Also provided are a population of microorganisms made by the provided methods. Methods of culturing the population of microorganisms and methods of reducing xylitol production in cultures comprising the population of microorganisms are provided.
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公开(公告)号：US10851395B2
公开(公告)日：2020-12-01
申请号：US15178890
申请日：2016-06-10
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/64
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.
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公开(公告)号：US10745642B2
公开(公告)日：2020-08-18
申请号：US15659300
申请日：2017-07-25
摘要： Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
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公开(公告)号：US20150337345A1
公开(公告)日：2015-11-26
申请号：US14719183
申请日：2015-05-21
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Provided herein are methods for producing one or more polyunsaturated fatty acids. The methods include the steps of providing a microorganism capable of producing polyunsaturated fatty acids, providing a medium comprising a high concentration of one or more carbon sources, low pH, or both, and culturing the microorganism in the medium under sufficient conditions to produce the one or more polyunsaturated fatty acids. Also provided are methods of culturing one or more microorganisms. The methods include culturing the microorganisms in a medium comprising a first amount of one or more carbon sources at a first concentration level, monitoring a carbon source concentration until the carbon source concentration is reduced below the first concentration level, and adding to the medium a second amount of one or more carbon sources to increase the carbon source concentration to a second concentration level.
摘要翻译： 本文提供了制备一种或多种多不饱和脂肪酸的方法。 所述方法包括提供能够产生多不饱和脂肪酸的微生物，提供包含高浓度一种或多种碳源，低pH或两者的培养基的步骤，以及在足够条件下在培养基中培养微生物以产生一种 或更多的多不饱和脂肪酸。 还提供培养一种或多种微生物的方法。 所述方法包括在包含第一量的一种或多种碳源的培养基中培养微生物，所述培养基以第一浓度水平监测碳源浓度，直到碳源浓度降低到低于第一浓度水平，并向培养基中加入第二 一个或多个碳源的量将碳源浓度增加到第二浓度水平。
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公开(公告)号：US20240060112A1
公开(公告)日：2024-02-22
申请号：US18490398
申请日：2023-10-19
IPC分类号： C12Q1/06 , C12N1/10 , C12N1/04 , C12P7/6409 , C12P7/6427
CPC分类号： C12Q1/06 , C12N1/10 , C12N1/04 , C12P7/6409 , C12P7/6427
摘要： Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.
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公开(公告)号：US20200332338A1
公开(公告)日：2020-10-22
申请号：US16864128
申请日：2020-04-30
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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公开(公告)号：US20200325465A1
公开(公告)日：2020-10-15
申请号：US16864098
申请日：2020-04-30
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
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公开(公告)号：US10676709B2
公开(公告)日：2020-06-09
申请号：US15655287
申请日：2017-07-20
发明人： Xuan Jiang , Dorothy Dennis , Roberto E. Armenta
摘要： Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.
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公开(公告)号：US20180208954A1
公开(公告)日：2018-07-26
申请号：US15926680
申请日：2018-03-20
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , C12P7/6463
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.
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