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公开(公告)号：US20240060112A1
公开(公告)日：2024-02-22
申请号：US18490398
申请日：2023-10-19
IPC分类号： C12Q1/06 , C12N1/10 , C12N1/04 , C12P7/6409 , C12P7/6427
CPC分类号： C12Q1/06 , C12N1/10 , C12N1/04 , C12P7/6409 , C12P7/6427
摘要： Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.
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公开(公告)号：US20200332338A1
公开(公告)日：2020-10-22
申请号：US16864128
申请日：2020-04-30
发明人： Zhiyong Sun , Michael Milway , Kevin Berryman , Mercia Valentine , Roberto E. Armenta , Laura Purdue
摘要： Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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公开(公告)号：US20200325465A1
公开(公告)日：2020-10-15
申请号：US16864098
申请日：2020-04-30
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
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公开(公告)号：US10676709B2
公开(公告)日：2020-06-09
申请号：US15655287
申请日：2017-07-20
发明人： Xuan Jiang , Dorothy Dennis , Roberto E. Armenta
摘要： Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.
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公开(公告)号：US20180208954A1
公开(公告)日：2018-07-26
申请号：US15926680
申请日：2018-03-20
发明人： Zhiyong Sun , Alan Sothern , Kevin Berryman , Mercia Valentine , Michael Milway , Laura Purdue , Roberto E. Armenta
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , C12P7/6463
摘要： Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.
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6.发明申请公开(公告)号：US20180179560A1
公开(公告)日：2018-06-28
申请号：US15845697
申请日：2017-12-18
IPC分类号： C12P7/64 , C12P23/00 , C12P21/00 , C12R1/89 , C12N1/12 , A23L33/135 , A23K50/00 , A23K10/18 , A23K20/147
CPC分类号： C12P7/6427 , A23K10/18 , A23K20/147 , A23K20/158 , A23K50/00 , A23L33/115 , A23L33/135 , A23V2002/00 , C12N1/10 , C12N1/12 , C12N1/14 , C12P7/6409 , C12P7/6472 , C12P21/00 , C12P23/00 , C12R1/645 , C12R1/89
摘要： Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.
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公开(公告)号：US20180023047A1
公开(公告)日：2018-01-25
申请号：US15655287
申请日：2017-07-20
发明人： Xuan Jiang , Dorothy Dennis , Roberto E. Armenta
摘要： Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.
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公开(公告)号：US20170321148A1
公开(公告)日：2017-11-09
申请号：US15659300
申请日：2017-07-25
摘要： Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
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公开(公告)号：US09745538B2
公开(公告)日：2017-08-29
申请号：US14577471
申请日：2014-12-19
CPC分类号： C11B1/10 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
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公开(公告)号：US20230416792A1
公开(公告)日：2023-12-28
申请号：US18220975
申请日：2023-07-12
IPC分类号： C12P7/6427 , C12P7/6463 , C12P7/6472 , C12P7/6434 , C12P7/6458 , C11B1/10
CPC分类号： C12P7/6427 , C12P7/6463 , C11B1/10 , C12P7/6434 , C12P7/6458 , C12P7/6472
摘要： Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
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