公开(公告)号：US20230348899A1

公开(公告)日：2023-11-02

申请号：US18333730

申请日：2023-06-13

申请人： Massachusetts Institute of Technology ,  The General Hospital Corporation

发明人： Peter van Galen ,  Volker Hovestadt ,  Travis Hughes ,  Marc H. Wadsworth II ,  Bradley Bernstein ,  Alexander K. Shalek ,  Todd M. Gierahn ,  J. Christopher Love ,  Ang A. Tu

IPC分类号： C12N15/10 ,  C12Q1/6886 ,  C12Q1/6888 ,  C12Q1/686

CPC分类号： C12N15/1065 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q1/6888 ,  C12Q2600/158

摘要： The present invention relates to methods of detecting region(s) of interest in a gene comprising a polyA tail. The region(s) of interest can include gene(s), region(s), mutation(s), deletion(s), insertion(s), indel(s), and/or translocation(s). The region(s) can be greater than or less than 1 kilobases from the polyA tail. Methods can include forming a library of single cell transcripts comprising the region(s) in close proximity to a cell barcode and a unique molecular identifier (UMI). Methods for distinguishing cells by genotype can include amplifying the transcripts using PCR methods and detecting the cell barcode and UMI using single cell sequencing methods. Transcripts can be enriched using tagged region-specific PCR primers. Cell barcodes can be brought into close proximity to the region(s) by circularizing the transcripts. Sequencing of the transcripts can include using primer binding sites added during PCR amplification and library indexes for multiplexed sequencing.

公开(公告)号：US20190144936A1

公开(公告)日：2019-05-16

申请号：US16070505

申请日：2017-01-17

申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Todd M. Gierahn ,  J. Christopher Love ,  Travis K. Hughes ,  Marc H. Wadsworth II ,  Alexander K. Shalek ,  Shaina Carroll

IPC分类号： C12Q1/6874 ,  B01J19/00

摘要： The present application provides a method of assembling a container for one or multiple parallel steps of biochemical analysis on one or more cells comprising performing molecular bonding of a porous membrane on an apical or basal surface of an array having a plurality of wells, wherein the molecular bonding substantially isolates each well from adjacent wells.

公开(公告)号：US20180100201A1

公开(公告)日：2018-04-12

申请号：US15844601

申请日：2017-12-17

申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  Dana-Farber Cancer Institute, Inc. ,  The General Hospital Corporation

发明人： Levi A. Garraway ,  Benjamin Izar ,  Sanjay Prakadan ,  Aviv Regev ,  Orit Rozenblatt-Rosen ,  Alexander K. Shalek ,  Mario Suva ,  Itay Tirosh ,  Andrew Venteicher ,  Marc H. Wadsworth II ,  Bradley Bernstein ,  Anuraag Parikh ,  Sidharth Puram

IPC分类号： C12Q1/6886

CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/158

摘要： This invention relates generally to compositions and methods for identifying genes and gene networks that respond to, modulate, control or otherwise influence tumors and tissues, including cells and cell types of the tumors and tissues, and malignant, microenvironmental, or immunologic states of the tumor cells and tissues. The invention also relates to methods of diagnosing, prognosing and/or staging of tumors, tissues and cells, and provides compositions and methods of modulating expression of genes and gene networks of tumors, tissues and cells, as well as methods of identifying, designing and selecting appropriate treatment regimens. The invention also relates to the modulation of complement activity to shift cellular immunity and obtain an effective therapeutic response.

公开(公告)号：US20200256862A1

公开(公告)日：2020-08-13

申请号：US16758504

申请日：2018-10-23

申请人： Massachusetts Institute of Technology

发明人： Alexander K. Shalek ,  Andrew Navia ,  Jeffrey F. van Humbeck ,  Marc H. Wadsworth II ,  Shaina Carroll ,  Travis K. Hughes

IPC分类号： G01N33/543 ,  C12Q1/6816

摘要： The present invention relates to functionalized solid supports and methods of making functionalized solid supports. Methods for preparing a population of high quality functionalized solid supports for use in various nucleic acid analysis methods are provided. The invention also provides methods for validation and quality control of the functionalization steps.

公开(公告)号：US20200248175A1

公开(公告)日：2020-08-06

申请号：US16758485

申请日：2018-10-23

申请人： Massachusetts Institute of Technology ,  The General Hospital Corporation

发明人： Peter van Galen ,  Volker Hovestadt ,  Travis Hughes ,  Marc H. Wadsworth II ,  Bradley Bernstein ,  Alexander K. Shalek ,  Todd M. Gierahn ,  J. Christopher Love ,  Ang A. Tu

IPC分类号： C12N15/10 ,  C12Q1/6888 ,  C12Q1/686 ,  C12Q1/6886

摘要： The present invention relates to methods of deriving genetic information from RNA-seq libraries, that can enable an overlay of genetic information (such as cancer driver mutations) onto single-cell transcriptomes and permitting efficient identification, localization, and quantification of certain cells of interest within a population as well as provide low-cost selection and sequencing of any portion of a transcript, including at the 5′ end.