公开(公告)号：US3922203A

公开(公告)日：1975-11-25

申请号：US38007073

申请日：1973-07-17

Applicant: MC DONNELL DOUGLAS CORP

Inventor： ALDRIDGE JR CLIFTON ,  VANNEST RICHARD D

IPC: C12Q1/04 ,  C12K1/04

CPC classification number: C12Q1/045 ,  Y10S435/808

Abstract: A cassette used to analyze a carrier liquid for the presence of a specific microorganism therein has a passageway extending through it and the passageway is provided with filter and viewing wells arranged such that each viewing well is preceded by a filter well. The carrier liquid is introduced into the passageway at one end thereof. Each filter well contains a filter material capable of effecting a one log reduction in the microorganisms within the liquid as the liquid passes through the filter. Each viewing cell contains plugs having a culture medium therein which is selective to a specific microorganism in the sense that only that microorganism will grow in the culture medium and change the light-transmitting characteristics of the viewing wells. To load the filter wells a pad of filter material is placed over the ends of the filter wells and the filters are simultaneously stamped from the pad and driven into the filter wells. To load the viewing wells, a flexible sheet of culture medium is placed over the ends of the viewing wells and the plugs are stamped therefrom and simultaneously driven into the viewing wells. The flexible sheet of culture medium is formed by adding a film-forming agent to liquid culture medium, pouring the solution so formed onto a surface, and then evaporating volatile components of the solution. After the volatile components evaporate, the flexible film remains.. The film-forming agent may be gelatin and glycerin, or it may be polyvinyl alcohol.

Abstract translation: 用于分析其中​​特定微生物存在的载体液体的盒具有延伸通过其的通道，并且通道设置有过滤器和观察孔，其布置成使得每个观察井之前都有过滤器孔。 载体液体在其一端被引入通道。 每个过滤器孔含有当液体通过过滤器时能够使液体内的微生物减少一个对数的过滤材料。 每个观察单元包含其中具有培养基的插塞，其中特定微生物是选择性的，只有该微生物将在培养基中生长并改变观察孔的透光特性。 为了装载过滤器孔，将过滤材料垫放置在过滤器孔的端部上，并且过滤器同时从垫冲压并被驱入过滤器孔。 为了加载观察井，将柔性培养基片放置在观察井的端部上，并且将塞子从其冲压并同时驱动到观察井中。 通过向液体培养基中添加成膜剂，将形成的溶液倾倒在表面上，然后蒸发溶液的挥发性成分，形成柔软的培养基片。 挥发性成分蒸发后，残留有柔性膜。成膜剂可以是明胶和甘油，也可以是聚乙烯醇。