公开(公告)号：US20220317082A1

公开(公告)日：2022-10-06

申请号：US17441169

申请日：2020-03-20

Applicant: MCMASTER UNIVERSITY

Inventor： Leyla SOLEYMANI ,  Richa PANDEY ,  Yingfu LI ,  Todd HOARE ,  Dingran CHANG ,  Yang LU

IPC: G01N27/327 ,  G01N33/543 ,  C12Q1/6804 ,  C12Q1/6825

Abstract: The present application relates to a biosensor for detecting an analyte comprising a first and second working electrode; a detection probe functionalized on the first working electrode, the detection probe comprising a reporter moiety and a recognition moiety for an analyte; a capture probe functionalized on the second working electrode; and a counter electrode. Each working electrode is configured to provide a change in signal if the analyte is present. The biosensor can be used to detect an analyte in a sample.

公开(公告)号：US20180363022A1

公开(公告)日：2018-12-20

申请号：US16060649

申请日：2016-12-12

Applicant: McMaster University

Inventor： Yingfu LI ,  John BRENNAN ,  Meng LIU

IPC: C12Q1/04 ,  C12Q1/686 ,  C12Q1/6806

Abstract: The present application is directed to biosensors and methods for detecting a microorganism target in a sample using a mechanically interlocked nucleic acid catanane, wherein an enzyme from the microorganism target or that is activated by a molecule from the microorganism target cleaves a linkage in a first single-stranded nucleic acid ring of the catanane structure, allowing rolling-circle amplification to occur and the presence of rolling-circle amplification products indicates the presence of the microorganism in the sample.