公开(公告)号：US20120129241A1

公开(公告)日：2012-05-24

申请号：US12909523

申请日：2010-10-21

申请人： MIN ZHANG ,  ARJUN SINGH ,  PIRKKO SUOMINEN ,  ERIC KNOSHAUG ,  MARY ANN FRANDEN ,  ERIC JARVIS

发明人： MIN ZHANG ,  ARJUN SINGH ,  PIRKKO SUOMINEN ,  ERIC KNOSHAUG ,  MARY ANN FRANDEN ,  ERIC JARVIS

IPC分类号： C12N1/19 ,  C12N15/31 ,  C12N15/81 ,  C07K14/39

CPC分类号： C12N15/81 ,  C12P7/06 ,  C12P7/08

摘要： An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.

摘要翻译： 提供利用酵母菌株的L-阿拉伯糖，用于通过引入和表达细菌araA，araB和araD基因来生产乙醇。 还将L-阿拉伯糖转运蛋白引入酵母中以增强阿拉伯糖的摄取。 酵母携带额外的基因组突变，使其能够消耗L-阿拉伯糖，甚至作为唯一的碳源，并产生乙醇。 还公开了通过新途径工程化代谢阿拉伯糖的酵母菌株。 生产乙醇的方法包括利用这些改良的酵母菌株。