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公开(公告)号：US07998722B2
公开(公告)日：2011-08-16
申请号：US12410501
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
CPC分类号： C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要： Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译： 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达，并且当该菌株另外被设计用于表达木酮糖激酶，转醛醇酶和转酮酶时，获得木糖的改善的利用。
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公开(公告)号：US08247208B2
公开(公告)日：2012-08-21
申请号：US12641642
申请日：2009-12-18
申请人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US20090246876A1
公开(公告)日：2009-10-01
申请号：US12410495
申请日：2009-03-25
申请人： PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , CAROL M. MCCUTCHEN , MARY ANN FRANDEN
发明人： PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , CAROL M. MCCUTCHEN , MARY ANN FRANDEN
CPC分类号： C12N15/74
摘要： Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
摘要翻译： 鉴定为运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的启动子的突变体，其直接改善可操作地连接的异源核酸的表达水平。 这些是用于在发酵单胞菌，酵母属和其他相关细菌中表达嵌合基因的高表达启动子。
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公开(公告)号：US08465961B2
公开(公告)日：2013-06-18
申请号：US13540641
申请日：2012-07-03
申请人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US08372626B2
公开(公告)日：2013-02-12
申请号：US12909523
申请日：2010-10-21
申请人： Min Zhang , Arjun Singh , Pirkko Suominen , Eric Knoshaug , Mary Ann Franden , Eric Jarvis
发明人： Min Zhang , Arjun Singh , Pirkko Suominen , Eric Knoshaug , Mary Ann Franden , Eric Jarvis
IPC分类号： C12N1/14
摘要： An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.
摘要翻译： 提供利用酵母菌株的L-阿拉伯糖，用于通过引入和表达细菌araA，araB和araD基因来生产乙醇。 还将L-阿拉伯糖转运蛋白引入酵母中以增强阿拉伯糖的摄取。 酵母携带额外的基因组突变，使其能够消耗L-阿拉伯糖，甚至作为唯一的碳源，并产生乙醇。 还公开了通过新途径工程化代谢阿拉伯糖的酵母菌株。 生产乙醇的方法包括利用这些改良的酵母菌株。
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公开(公告)号：US20120129241A1
公开(公告)日：2012-05-24
申请号：US12909523
申请日：2010-10-21
申请人： MIN ZHANG , ARJUN SINGH , PIRKKO SUOMINEN , ERIC KNOSHAUG , MARY ANN FRANDEN , ERIC JARVIS
发明人： MIN ZHANG , ARJUN SINGH , PIRKKO SUOMINEN , ERIC KNOSHAUG , MARY ANN FRANDEN , ERIC JARVIS
摘要： An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.
摘要翻译： 提供利用酵母菌株的L-阿拉伯糖，用于通过引入和表达细菌araA，araB和araD基因来生产乙醇。 还将L-阿拉伯糖转运蛋白引入酵母中以增强阿拉伯糖的摄取。 酵母携带额外的基因组突变，使其能够消耗L-阿拉伯糖，甚至作为唯一的碳源，并产生乙醇。 还公开了通过新途径工程化代谢阿拉伯糖的酵母菌株。 生产乙醇的方法包括利用这些改良的酵母菌株。
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公开(公告)号：US07989206B2
公开(公告)日：2011-08-02
申请号：US12410495
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Laura McCole , Min Zhang , Yat-Chen Chou , Carol M. McCutchen , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Laura McCole , Min Zhang , Yat-Chen Chou , Carol M. McCutchen , Mary Ann Franden
CPC分类号： C12N15/74
摘要： Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
摘要翻译： 鉴定为运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的启动子的突变体，其直接改善可操作地连接的异源核酸的表达水平。 这些是用于在发酵单胞菌，酵母属和其他相关细菌中表达嵌合基因的高表达启动子。
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8.发明申请公开(公告)号：US20110014670A1
公开(公告)日：2011-01-20
申请号：US12641642
申请日：2009-12-18
申请人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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9.发明授权Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate 有权在含有浓缩糖和乙酸盐的培养基中乙醇产量提高的发酵单胞菌公开(公告)号：US07803623B2
公开(公告)日：2010-09-28
申请号：US12261166
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
CPC分类号： C12N15/52 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12N15/74 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。
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公开(公告)号：US20090246846A1
公开(公告)日：2009-10-01
申请号：US12410501
申请日：2009-03-25
申请人： PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , CAROL M. MCCUTCHEN , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , MARY ANN FRANDEN
发明人： PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , CAROL M. MCCUTCHEN , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , MARY ANN FRANDEN
CPC分类号： C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要： Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译： 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达，并且当该菌株另外被设计用于表达木酮糖激酶，转醛醇酶和转酮酶时，获得木糖的改善的利用。
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